New thermolytic carbamoyl groups for the protection of nucleobases

Autor:	Akihiro Ohkubo, Rintaro Kasuya, Kohji Seio, Mitsuo Sekine, Hirosuke Tsunoda, Kenichi Miyata
Rok vydání:	2009
Předmět:	Hot Temperature Deoxyadenosines Chemistry Stereochemistry Hybridization probe Organic Chemistry Nucleic Acid Precursors Reversible process Biochemistry Deoxycytidine Nucleobase Carbamates Physical and Theoretical Chemistry Deoxyadenosine Derivatives DNA Probes
Zdroj:	Organicbiomolecular chemistry. 7(4)
ISSN:	1477-0539
Popis:	It was found that N-arylcarbamoyl and N-(phenylsulfonyl)carbamoyl (psc) groups could be effectively introduced onto the amino groups of deoxycytidine and deoxyadenosine derivatives and could be removed thermolytically. We succeeded in synthesizing DNA probes incorporating these thermo-removable protecting groups and developed a new system for molecular switching by changing the protection- and deprotection-modes using simple heating and re-carbamoylation with isocyanates. This reversible process enabled us to control the hybridization ability of the DNA probes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::796153db3d39e8f194854f2b3ed6f89a https://pubmed.ncbi.nlm.nih.gov/19194584 Zobrazit plný text záznamu