Development and comparative analysis of yeast protein extraction protocols for mass spectrometry
| Autor: | Lauana Fogaça de Almeida, Lucilene Delazari dos Santos, Guilherme Targino Valente, Leonardo Nazario de Moraes |
|---|---|
| Přispěvatelé: | Universidade Estadual Paulista (Unesp) |
| Rok vydání: | 2019 |
| Předmět: |
Proteomics
Saccharomyces cerevisiae Proteins Liquid-Liquid Extraction Biophysics Saccharomyces cerevisiae Saccharomyces cerevisiae proteins Mass spectrometry 01 natural sciences Biochemistry 03 medical and health sciences Protocol comparisons Tandem Mass Spectrometry Protein purification Molecular Biology 030304 developmental biology Protocol (science) 0303 health sciences Chromatography Chemistry 010401 analytical chemistry Extraction (chemistry) Temperature Cell Biology Yeast 0104 chemical sciences Protein extraction protocol Protein identification |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/j.ab.2018.10.028 |
| Popis: | Made available in DSpace on 2019-10-06T16:11:18Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-02-15 Universidade Estadual Paulista Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Mass spectrometry is the most used method for protein identification and quantification. Here we developed four protein extraction protocols precisely for mass spectrometry, and we compared with other ones already published. The best protocol developed by us consists on a simple extraction solution, a heat-shock step, and does not use protease inhibitor; moreover, it is the most efficient and uniform among replicates, besides to be safe, cheap and fast. That method also provided the highest number of proteins uniquely identified and allows finding a diversity of protein classes, which their absence is a problem to be avoided. Sao Paulo State University (UNESP) Department of Bioprocess and Biotechnology School of Agriculture, Avenue Universitária, n 3780, Altos do Paraíso Sao Paulo State University (UNESP) Center for the Study of Venoms and Venomous Animals (CEVAP), Avenue Universitária, n 3780, Altos do Paraíso São Paulo State University (UNESP) Graduate Program in Tropical Diseases, Botucatu Medical School (FMB). Avenue Prof. Mário Rubens Guimarães Montenegro, no number, Rubião Jr Sao Paulo State University (UNESP) Department of Bioprocess and Biotechnology School of Agriculture, Avenue Universitária, n 3780, Altos do Paraíso Sao Paulo State University (UNESP) Center for the Study of Venoms and Venomous Animals (CEVAP), Avenue Universitária, n 3780, Altos do Paraíso São Paulo State University (UNESP) Graduate Program in Tropical Diseases, Botucatu Medical School (FMB). Avenue Prof. Mário Rubens Guimarães Montenegro, no number, Rubião Jr Universidade Estadual Paulista: 12/2015-PROPe FAPESP: 2015/12093-9 |
| Databáze: | OpenAIRE |
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