Erratum to 'Adaptive responses of Bacillus cereus ATCC14579 cells upon exposure to acid conditions involve ATPase activity to maintain their internal pH'

Autor: Michel Jobin, Khadidja Senouci-Rezkallah, Philippe Schmitt
Přispěvatelé: Sécurité et Qualité des Produits d'Origine Végétale (SQPOV), Avignon Université (AU)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Mascara, Université de Montréal (UdeM), Senouci, Khadidja, Senouci-Rezkallah, Khadidja
Jazyk: angličtina
Rok vydání: 2015
Předmět:
F1F0-ATPase
Nigericin
analyse stastique
ATPase
health care facilities
manpower
and services
[SDV]Life Sciences [q-bio]
education
Bacillus cereus
atpB gene expression
atpase f1f0
Biology
Microbiology
pHi
choc acide
03 medical and health sciences
chemistry.chemical_compound
Valinomycin
ionophores
0404 agricultural biotechnology
[SDV.IDA]Life Sciences [q-bio]/Food engineering
entérotoxine
[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering
health care economics and organizations
030304 developmental biology
Original Research
chemistry.chemical_classification
N
N-Dicyclohexylcarbodiimide
0303 health sciences
chemostat
fungi
syndrome émétique
04 agricultural and veterinary sciences
biology.organism_classification
040401 food science
pH i
Enzyme
N
N'-dicyclohexylcarbodiimide
Biochemistry
chemistry
Cereus
syndrome diarrhéique
biology.protein
bacteria
N
N’-dicyclohexylcarbodiimide
arn messager
Corrigendum
Homeostasis
Zdroj: MicrobiologyOpen
MicrobiologyOpen, Wiley, 2015, 4 (6), pp.1001. ⟨10.1002/mbo3.317⟩
MicrobiologyOpen, Wiley, 2015, 4 (2), pp.313-322. ⟨10.1002/mbo3.239⟩
MicrobiologyOpen 6 (4), 1001. (2015)
MicrobiologyOpen 2 (4), 313-322. (2015)
ISSN: 2045-8827
DOI: 10.1002/mbo3.317⟩
Popis: International audience; This study examined the involvement of ATPase activity in the acid tolerance response (ATR) of Bacillus cereus ATCC14579 strain. In the current work, B. cereus cells were grown in anaerobic chemostat culture at external pH (pHe) 7.0 or 5.5 and at a growth rate of 0.2 h -1. Population reduction and internal pH (pHi) after acid shock at pH 4.0 was examined either with or without ATPase inhibitor N,N’-dicyclohexylcarbodiimide (DCCD) and ionophores valinomycin and nigericin. Population reduction after acid shock at pH 4.0 was strongly limited in cells grown at pH 5.5 (acid-adapted cells) compared with cells grown at pH 7.0 (unadapted cells), indicating that B. cereus cells grown at low pHe were able to induce a significant ATR and Exercise-induced increase in ATPase activity. However, DCCD and ionophores had a negative effect on the ability of B. cereus cells to survive and maintain their pHi during acid shock. When acid shock was achieved after DCCD treatment, pHi was markedly dropped in unadapted and acid-adapted cells. The ATPase activity was also significantly inhibited by DCCD and ionophores in acid-adapted cells. Furthermore, transcriptional analysis revealed that atpB (ATP beta chain) transcripts was increased in acid-adapted cells compared to unadapted cells before and after acid shock. Our data demonstrate that B. cereus is able to induce an ATR during growth at low pH. These adaptations depend on the ATPase activity induction and pHi homeostasis. Our data demonstrate that the ATPase enzyme can be implicated in the cytoplasmic pH regulation and in acid tolerance of B. cereus acid-adapted cells.
Databáze: OpenAIRE
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