A modified version of the digestion–ligation cloning method for more efficient molecular cloning
| Autor: | Jiannan Zhang, Hongman Chen, Daming Ren, Yanling Li, Song Gao, Yingfeng An, Lijun Zhang |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Cloning
chemistry.chemical_classification DNA ligase DNA Ligases Genetic Vectors Biophysics DNA DNA Restriction Enzymes Cell Biology Computational biology Molecular cloning Biology Biochemistry Molecular biology Insert (molecular biology) Sequencing by ligation chemistry.chemical_compound chemistry Vector (molecular biology) Cloning Molecular Genetic Engineering Ligation Molecular Biology |
| Zdroj: | Analytical Biochemistry. 453:55-57 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/j.ab.2014.02.031 |
| Popis: | Here we describe a modified version of the digestion-ligation approach for efficient molecular cloning. In comparison with the original method, the modified method has the additional steps of gel purification and a second ligation after the first ligation of the linearized vector and DNA insert. During this process, the efficiency and reproducibility could be significantly improved for both stick-end cloning and blunt-end cloning. As an improvement of the very important molecular cloning technique, this method may find a wide range of applications in bioscience and biotechnology. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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