A Partially Protective Vaccine for Fasciola hepatica Induced Degeneration of Adult Flukes Associated to a Severe Granulomatous Reaction in Sheep
| Autor: | Verónica Molina-Hernández, José Pérez, M.J. Bautista, Raúl Pérez-Caballero, María Teresa Ruiz-Campillo, Francisco Javier Martinez-Moreno, Álvaro Martínez-Moreno, L. Buffoni, A. Escamilla, Rafael Zafra |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Pathology
medicine.medical_specialty sheep 040301 veterinary sciences Veterinary medicine CD3 Histopathology Article 0403 veterinary science Fasciola hepatica 03 medical and health sciences Multinucleate SF600-1100 parasitic diseases Medicine Fasciolosis 030304 developmental biology 2. Zero hunger 0303 health sciences Vaccines Sheep General Veterinary biology business.industry CD68 Vaccine trial 04 agricultural and veterinary sciences vaccines medicine.disease biology.organism_classification Immunohistochemistry 3. Good health QL1-991 Giant cell immunohistochemistry biology.protein histopathology Animal Science and Zoology business Zoology Infiltration (medical) |
| Zdroj: | Animals Volume 11 Issue 10 Animals 11(10), 2869 (2021) Animals : an Open Access Journal from MDPI Animals, Vol 11, Iss 2869, p 2869 (2021) Helvia. Repositorio Institucional de la Universidad de Córdoba instname |
| ISSN: | 2076-2615 |
| DOI: | 10.3390/ani11102869 |
| Popis: | Simple Summary Fasciolosis is a parasitic disease of livestock causing important economic losses worldwide and it is also a zoonosis. Current therapy relies on the use of anthelmintic drugs, which is no longer sustainable due to the increase of anthelmintic resistance and the risk of drug residues in food. A deep understanding of the host-parasite interaction is required to develop protective vaccines for the control of fasciolosis. The aim of the present study is to evaluate the hepatic lesions in sheep vaccinated with a partly protective vaccine for F. hepatica, a non-protective vaccine and an infected control group. The protective vaccine showed less severe hepatic lesions than the infected control group. In addition, in the protective vaccine group dead flukes surrounded by a severe granulomatous inflammation were observed, which taken together with the lower fluke burden, suggests that the host response induced by the partially protective vaccine may have been involved in the death of adult flukes of F. hepatica. This is the first study reporting the presence of degenerated flukes associated to a severe granulomatous inflammation in bile ducts in a vaccine trial, a finding that would be useful for improving vaccine efficacy in future trials. Abstract Fasciolosis is an important economic disease of livestock. There is a global interest in the development of protective vaccines since current anthelmintic therapy is no longer sustainable. A better knowledge of the host-parasite interaction is needed for the design of effective vaccines. The present study evaluates the microscopical hepatic lesions in sheep immunized with a partially protective vaccine (VAC1), a non-protective vaccine (VAC2), and an infected control group (IC). The nature of granulomatous inflammation associated with degeneration of adult flukes found in the VAC1 group was characterized by immunohistochemistry. Hepatic lesions (fibrous perihepatitis, chronic tracts, bile duct hyperplasia, infiltration of eosinophils and lymphocytes and plasma cells) were significantly less severe in the VAC1 group than in the IC group. Dead adult flukes within bile ducts were observed only in the VAC1 group and were surrounded by a severe granulomatous inflammation composed by macrophages and multinucleate giant cells with a high expression of lysozyme, CD163 and S100 markers, and a low expression of CD68. Numerous CD3+ T lymphocytes and scarce infiltrate of FoxP3+ Treg and CD208+ dendritic cells were present. This is the first report describing degenerated flukes associated to a severe granulomatous inflammation in bile ducts in a F. hepatica vaccine trial. |
| Databáze: | OpenAIRE |
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