Vasocontractile muscarinic M1 receptors in cat cerebral arteries: pharmacological identification and detection of mRNA
| Autor: | Vincent Ting, Michael Dennis, Edith Hamel, François Dauphin, Paul Payette |
|---|---|
| Přispěvatelé: | DAUPHIN, François, Groupe Mémoire et Plasticité comportementale (GMPc), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), Mobilités : Vieillissement, Pathologie, Santé (COMETE), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Caen Normandie (UNICAEN), Montreal Neurological Institute and Hospital, McGill University = Université McGill [Montréal, Canada], Conseil National de Recherches Canada (CNRC), Iowa State University (ISU) |
| Jazyk: | angličtina |
| Rok vydání: | 1991 |
| Předmět: |
Atropine
Male [SDV.BA] Life Sciences [q-bio]/Animal biology MESH: Muscle Contraction Muscle Smooth Vascular MESH: Nucleic Acid Hybridization chemistry.chemical_compound 0302 clinical medicine Muscarinic acetylcholine receptor Muscarinic acetylcholine receptor M5 Muscarinic acetylcholine receptor M4 Methoctramine Vasoconstrictor Agents MESH: Animals MESH: Cerebral Arteries 0303 health sciences MESH: Kinetics Muscarinic receptor subtypes [SDV.BA]Life Sciences [q-bio]/Animal biology Nucleic Acid Hybridization Muscarinic acetylcholine receptor M3 Muscarinic acetylcholine receptor M2 Muscarinic acetylcholine receptor M1 MESH: Muscle Smooth Vascular [SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences Muscarinic M1 receptor Receptors Muscarinic MESH: Muscarinic Antagonists 3. Good health [SDV.SP] Life Sciences [q-bio]/Pharmaceutical sciences Female [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] MESH: Endothelium Vascular MESH: Cats Muscle Contraction medicine.drug medicine.medical_specialty Cerebral blood vessels (cat) Muscarinic Antagonists [SDV.BC]Life Sciences [q-bio]/Cellular Biology In Vitro Techniques Biology 03 medical and health sciences MESH: Vasoconstrictor Agents MESH: Atropine Internal medicine medicine Animals MESH: Blotting Northern RNA Messenger [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] [SDV.BC] Life Sciences [q-bio]/Cellular Biology 030304 developmental biology MESH: RNA Messenger Pharmacology MESH: In Vitro Techniques MESH: Acetylcholine Muscarinic receptor antagonists RNA Probes Cerebral Arteries Blotting Northern Pirenzepine Acetylcholine MESH: Male Kinetics Endocrinology chemistry MESH: Receptors Muscarinic Vasoconstriction Cats MESH: RNA Probes Endothelium Vascular MESH: Female 030217 neurology & neurosurgery |
| Zdroj: | European Journal of Pharmacology European Journal of Pharmacology, Elsevier, 1991, 207 (4), pp.319-27 |
| ISSN: | 0014-2999 |
| Popis: | International audience; The nature of the muscarinic receptor subtype mediating the acetylcholine (ACh)-induced constriction of the cat middle cerebral artery was investigated in vitro by recording the smooth muscle isometric tension of precontracted endothelium-denuded arterial segments. The ability of selective (pirenzepine, UH-AH 371, AF-DX 116, methoctramine, AQ-RA 741, 4-diphenylacetoxy-N-methylpiperidine methiodide (4-DAMP) and hexahydro-sila-difenidol (HHSiD)) and non-selective (atropine) antagonists to inhibit the constriction elicited by ACh was estimated. In addition, using a subtype-specific ribonucleotide probe directed against mRNA encoding the human m1 (Hm1) muscarinic receptor, identification of the corresponding vascular receptor was undertaken in total RNA extracts from cat cerebral blood vessels. The potent inhibition of the ACh-induced constriction by M1 antagonists (pirenzepine and UH-AH 371; pA2 values respectively of 8.08 and 8.64), together with lower affinities of M2 (AF-DX 116; pA2 = 6.50, methoctramine; pA2 = 6.27 and AQ-RA 741; pA2 = 7.60) and M3 compounds (4-DAMP and HHSiD; with pA2 values of 8.85 and 7.76, respectively) strongly suggested the involvement of a pharmacological M1 receptor in this vasomotor response. Furthermore, Northern blot hybridization with the selective Hm1 ribonucleotide probe showed the presence of mRNA transcripts for this muscarinic receptor subtype in the cat cerebrovascular bed. The results indicate that muscarinic constriction in the feline cerebrovascular bed is mediated by a pharmacological M1 receptor subtype and that the corresponding m1 receptor mRNA is present in cat cerebral blood vessels. These findings clearly point to a role of M1 muscarinic receptors in cerebrovascular function. |
| Databáze: | OpenAIRE |
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