Modelling Cryptosporidium infection in human small intestinal and lung organoids
| Autor: | Kim E Boonekamp, Antoni P. A. Hendrickx, Benedetta Artegiani, Deborah A. Schaefer, Michael W. Riggs, Inha Heo, Devanjali Dutta, Robert J L Willems, Nino Iakobachvili, Roberta M. O'Connor, Gregory D. Bowden, Hans Clevers, Norman Sachs, Peter J. Peters |
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| Přispěvatelé: | Hubrecht Institute for Developmental Biology and Stem Cell Research, Institute of Nanoscopy (IoN), RS: M4I - Nanoscopy, School Office GROW |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Cryptosporidium infection PARVUM SPOROZOITES Immunology Disease Biology Microbiology Applied Microbiology and Biotechnology DISEASE Transcriptome CULTURE 03 medical and health sciences parasitic diseases INFECTION medicine Organoid Genetics Parasite hosting LISTERIA-MONOCYTOGENES ELECTRON-MICROSCOPIC OBSERVATION Lung CRYPTOSPORIDIOSIS MORTALITY ZAMBIAN CHILDREN Cryptosporidium Cell Biology APICOMPLEXA biology.organism_classification medicine.disease 3. Good health 030104 developmental biology medicine.anatomical_structure Drug development |
| Zdroj: | Nature Microbiology, 3(7), 814-823. Nature Publishing Group Nature Microbiology, 3(7), 814. Nature Publishing Group Nature Microbiology |
| ISSN: | 2058-5276 |
| Popis: | Stem-cell-derived organoids recapitulate in vivo physiology of their original tissues, representing valuable systems to model medical disorders such as infectious diseases. Cryptosporidium, a protozoan parasite, is a leading cause of diarrhoea and a major cause of child mortality worldwide. Drug development requires detailed knowledge of the pathophysiology of Cryptosporidium, but experimental approaches have been hindered by the lack of an optimal in vitro culture system. Here, we show that Cryptosporidium can infect epithelial organoids derived from human small intestine and lung. The parasite propagates within the organoids and completes its complex life cycle. Temporal analysis of the Cryptosporidium transcriptome during organoid infection reveals dynamic regulation of transcripts related to its life cycle. Our study presents organoids as a physiologically relevant in vitro model system to study Cryptosporidium infection. |
| Databáze: | OpenAIRE |
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