Untargeted LC-MS Metabolomics for the Analysis of Micro-scaled Extracellular Metabolites from Hepatocytes
| Autor: | Rodi Abdalkader, Pei Zhang, Ken-ichiro Kamei, Craig E. Wheelock, Isabel Meister, Romanas Chaleckis |
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| Rok vydání: | 2020 |
| Předmět: |
Metabolite
010401 analytical chemistry 02 engineering and technology 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences Analytical Chemistry Low volume chemistry.chemical_compound medicine.anatomical_structure Metabolomics Biochemistry chemistry Tandem Mass Spectrometry Cell culture Liquid chromatography–mass spectrometry Hepatocyte Hepatocytes Metabolome medicine Extracellular 0210 nano-technology Chromatography Liquid |
| Zdroj: | Analytical Sciences. 37:1049-1052 |
| ISSN: | 1348-2246 0910-6340 |
| Popis: | Metabolome analysis in micro physiological models is a challenge due to the low volume of cell culture medium (CCM). Here, we report a LC-MS-based untargeted metabolomics protocol for the detection of hepatocyte extracellular metabolites from micro-scale samples of CCM. Using a single LC-MS method we have detected 57 metabolites of which 27 showed >2-fold shifts after 72-hours incubation. We demonstrate that micro-scale CCM samples can be used for modelling micro-physiological temporal dynamics in metabolite intensities.Graphical Abstract |
| Databáze: | OpenAIRE |
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