Kaposi's Sarcoma-Associated Herpesvirus Open Reading Frame 50/Rta Protein Activates the Entire Viral Lytic Cycle in the HH-B2 Primary Effusion Lymphoma Cell Line†
| Autor: | Craig Metroka, Duane Shedd, L Gradoville, Sarah Nikiforow, Elizabeth Grogan, George Miller, Jennifer Gerlach |
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| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
Gene Expression Regulation
Viral Phosphonoacetic Acid Lymphoma viruses Immunology Replication Biology medicine.disease_cause Microbiology Immediate early protein Immediate-Early Proteins Gene product Virology medicine Tumor Cells Cultured Humans RNA Messenger Kaposi's sarcoma-associated herpesvirus Regulation of gene expression Expression vector Deoxyribonucleases virus diseases Transfection biochemical phenomena metabolism and nutrition medicine.disease Molecular biology Lytic cycle Insect Science DNA Viral Herpesvirus 8 Human Trans-Activators Reverse Transcriptase Inhibitors Primary effusion lymphoma |
| Popis: | Rta, the gene product of Kaposi's sarcoma-associated herpesvirus (KSHV) encoded mainly in open reading frame 50 (ORF50), is capable of activating expression of viral lytic cycle genes. What was not demonstrated in previous studies was whether KSHV Rta was competent to initiate the entire viral lytic life cycle including lytic viral DNA replication, late-gene expression with appropriate kinetics, and virus release. In HH-B2, a newly established primary effusion lymphoma (PEL) cell line, KSHV ORF50 behaved as an immediate-early gene and autostimulated its own expression. Expression of late genes, ORF65, and K8.1 induced by KSHV Rta was eliminated by phosphonoacetic acid, an inhibitor of viral DNA polymerase. Transfection of KSHV Rta increased the production of encapsidated DNase-resistant viral DNA from HH-B2 cells. Thus, introduction of an ORF50 expression plasmid is sufficient to drive the lytic cycle to completion in cultured PEL cells. |
| Databáze: | OpenAIRE |
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