Leptospira spp. infection in sheep herds in southeast Brazil
| Autor: | Priscila Barbante, Helio Langoni, Fabio Hiroto Shimabukuro, Simone Baldini Lucheis, Virgínia Bodelão Richini-Pereira |
|---|---|
| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Instituto Adolfo Lutz (IAL), Universidade de São Paulo (USP) |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Serotype
Veterinary medicine lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 Culture Context (language use) Toxicology law.invention Serology Leptospira law lcsh:RA1190-1270 Direct agglutination test lcsh:Zoology Medicine Leptospirosis lcsh:QL1-991 Polymerase chain reaction lcsh:Toxicology. Poisons biology business.industry Research biology.organism_classification medicine.disease Ovine Infectious Diseases PCR Herd Animal Science and Zoology Parasitology business |
| Zdroj: | Journal of Venomous Animals and Toxins including Tropical Diseases v.20 2014 The Journal of venomous animals and toxins including tropical diseases Universidade Estadual Paulista (UNESP) instacron:UNESP The Journal of Venomous Animals and Toxins Including Tropical Diseases Journal of Venomous Animals and Toxins including Tropical Diseases, Volume: 20, Pages: 15-9, Published: 06 JUN 2014 Web of Science Repositório Institucional da UNESP Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 20, Iss 0, Pp 9-15 (2014) |
| Popis: | Made available in DSpace on 2014-12-03T13:10:38Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-05-06Bitstream added on 2014-12-03T13:24:35Z : No. of bitstreams: 1 WOS000335792300002.pdf: 230343 bytes, checksum: 990b70d175a4215dea8bca546583875d (MD5) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Regional Laboratory of Sorocaba - Adolfo Lutz Institute Background: With the aim of studying Leptospira spp. infection in sheep herds, blood samples and respective kidney and liver fragments were collected from 100 animals from twenty different properties during slaughter at a meat company in the Sorocaba region, Sao Paulo state, southeast Brazil. The microscopic agglutination test (MAT) was performed with 29 strains of Leptospira spp. To identify the agent in the liver and kidney, 100 samples of each tissue were submitted to culture in Fletcher medium and analyzed by the polymerase chain reaction (PCR) for Leptospira spp.Results: MAT detected 23 samples serologically positive for one or more Leptospira spp. serovars and significantly more for Autumnalis. Eight (4%) samples were positive in culture (four kidneys and four livers), corresponding to five animals with positive serology (one animal simultaneously positive for both kidney and liver) and two negatives. PCR detected Leptospira spp. in 14 samples (seven kidneys and seven livers) corresponding to 12 positive animals (two animals simultaneously positive for kidney and liver), of which ten were serologically positive and two negative.Conclusions: PCR was faster, more practical and more sensitive than culture for detecting leptospires. The results reinforce the importance of sheep in the epidemiological context of leptospirosis. Univ Estadual Paulista, Sao Paulo State Univ, UNESP, Sch Vet Med & Anim Husb,Dept Vet Hyg & Publ Hlth, Botucatu, SP, Brazil Adolfo Lutz Inst, Sorocaba, SP, Brazil Adolfo Lutz Inst CLR II, Bauru, SP, Brazil Paulista Agcy Agribusiness Technol APTA SAA, BR-17030000 Bauru, SP, Brazil Univ Estadual Paulista, Sao Paulo State Univ, UNESP, Sch Vet Med & Anim Husb,Dept Vet Hyg & Publ Hlth, Botucatu, SP, Brazil FAPESP: 08/52537-0 FAPESP: 08/53714-2 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|