The effect of wortmannin on radiation-induced chromosome aberration formation in the radioresistant tumor cell line WiDr
| Autor: | Heinz Schmidberger, Margret Rave-Fränk, Hana Hofman-Hüther, Patricia Virsik-Köpp |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
DNA damage
Biophysics DNA-Activated Protein Kinase Biology Protein Serine-Threonine Kinases Chromosome aberration Radiation Tolerance Resting Phase Cell Cycle Wortmannin 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cell Line Tumor Humans Radiology Nuclear Medicine and imaging Metaphase 030304 developmental biology Chromosome Aberrations 0303 health sciences Radiation Dose-Response Relationship Drug G1 Phase Nuclear Proteins Cell cycle Molecular biology Androstadienes DNA-Binding Proteins chemistry Cell culture 030220 oncology & carcinogenesis Colonic Neoplasms Chromatid Homologous recombination DNA Damage |
| Zdroj: | Radiation research. 164(2) |
| ISSN: | 0033-7587 |
| Popis: | We analyzed the formation of radiation-induced chromosome aberrations in the cells of the radioresistant colon carcinoma cell line WiDr after treatment with wortmannin, an inhibitor of PI-3 kinases, including DNA-PK. Cells irradiated in G0/G1 phase with 200 kV X rays were treated with wortmannin before or after irradiation. Chromosome-type and chromatid-type aberrations were scored in metaphase cells by either Giemsa staining or FISH. Moreover, DNA-PK activity was measured in the absence and presence of wortmannin. In irradiated G0/G1-phase WiDr cells, only chromosome-type aberrations, including simple and complex exchanges and excess acentrics, were observed. After addition of 1 to 20 microM wortmannin, the formation of chromosome-type exchange aberrations was completely suppressed. The irradiated cells displayed exclusively chromatid-type aberrations including simple and complex chromatid exchanges and chromatid/isochromatid breaks. Whether the chromatid-type aberrations arise during G0/G1 as a result of homologous recombination processes coping with damaged DNA or whether DNA damage induced during G0/G1 phase persists until S and G2 phase and is then processed by homologous recombination pathways must be investigated further. |
| Databáze: | OpenAIRE |
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