Detection and distribution of vbnc/viable pathogenic bacteria in full-scale drinking water treatment plants
| Autor: | Chengsong Ye, Kassim Chabi, Xin Yu, Kun Wan, Jianwen Zhu, Lizheng Guo |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Salmonella
Environmental Engineering Health Toxicology and Mutagenesis 0211 other engineering and technologies 02 engineering and technology 010501 environmental sciences Biology medicine.disease_cause 01 natural sciences Water Purification Microbiology Propidium monoazide RNA Ribosomal 16S Escherichia coli medicine Environmental Chemistry Waste Management and Disposal 0105 earth and related environmental sciences 021110 strategic defence & security studies Bacteria Drinking Water Biofilm Pathogenic bacteria Sphingomonas biology.organism_classification Pollution Water treatment Mycobacterium |
| Zdroj: | Journal of Hazardous Materials. 406:124335 |
| ISSN: | 0304-3894 |
| DOI: | 10.1016/j.jhazmat.2020.124335 |
| Popis: | Viable but non-culturable (VBNC) bacteria have attracted widespread attention since they are inherently undetected by traditional culture-dependent methods. Importantly, VBNC bacteria could resuscitate under favorable conditions leading to significant public health concerns. Although the total number of viable bacteria has been theorized to be far greater than those that can be cultured, there have been no reports quantifying VBNC pathogenic bacteria in full-scale drinking water treatment plants (DWTPs). In this work, we used both culture-dependent and quantitative PCR combination with propidium monoazide (PMA) dye approaches to characterize cellular viability. Further, we established a method to quantify viable pathogens by relating specific gene copies to viable cell numbers. Ratios of culturable bacteria to viable 16S rRNA gene copies in water and biological activated carbon (BAC) biofilms were 0–4.75% and 0.04–56.24%, respectively. The VBNC E. coli, E. faecalis, P. aeruginosa, Salmonella sp., and Shigella sp. were detected at levels of 0–103 cells/100 mL in source water, 0–102 cells/100 mL in chlorinated water, and 0–103 cells/g in BAC biofilms. In addition, differences between the total and viable community structures after ozonation and chlorination were investigated. The relative abundance of opportunistic pathogens such as Mycobacterium, Sphingomonas, etc. increased in final water, likely due to their chlorine resistance. In summary, we detected significant quantities of viable/VBNC opportunistic pathogens in full-scale DWTPs, confirming that traditional, culture-dependent methods are inadequate for detecting VBNC bacteria. These findings suggest a need to develop and implement rapid, accurate methods for the detection of VBNC pathogenic bacteria in DWTPs to ensure the safety of drinking water. |
| Databáze: | OpenAIRE |
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