Class C1 decoy oligodeoxynucleotide inhibits profibrotic genes expression in rat hepatic stellate cells
| Autor: | Jiang‑Feng Wu, Lin Han, Yi‑Ran Ni, Rui‑Ting Zhou, Yan‑Min Zhao, Chang-Bai Liu, Chun Rao, Yan‑Qiong Zhang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
transforming growth factor-β signaling Liver Cirrhosis Cancer Research Down-Regulation class C1 decoy oligodeoxynucleotides Biochemistry Collagen Type I Cell Line 03 medical and health sciences 0302 clinical medicine Transforming Growth Factor beta Gene expression Genetics Hepatic Stellate Cells Animals Smad3 Protein Promoter Regions Genetic Molecular Biology TIMP1 Tissue Inhibitor of Metalloproteinase-1 Chemistry hemic and immune systems Transfection Articles Molecular biology Rats Blot 030104 developmental biology Oncology Gene Expression Regulation Oligodeoxyribonucleotides Cell culture 030220 oncology & carcinogenesis Hepatic stellate cell Molecular Medicine Transcription Factor HES-1 Decoy Transforming growth factor |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 |
| Popis: | The aim of the present study was to investigate whether class C1 decoy oligodeoxynucleotides (ODNs) can inhibit the expression of pro‑fibrotic genes associated with rat hepatic stellate cell (HSC) activation and hepatic fibrosis. Luciferase reporter assays were performed to test the promoter activities of transforming growth factor (TGF)‑β and its downstream target genes following transfection of decoy ODNs and plasmids into HSC‑T6 cells, and western blot assays were performed to measure the protein expression of those genes following decoy ODN transfection. Class C1 decoy ODNs were confirmed to inhibit the promoter activity of TGF‑β and its downstream target genes, such as type 1 collagen (COLI)α1, tissue inhibitor of metalloproteinases (TIMP)1 and α‑smooth muscle actin by Gaussia luciferase reporter assay, and to further downregulate the expression of TGF‑β, SMAD3, COLIα1 and TIMP1 by western blotting in activated HSC‑T6 cells. In conclusion, class C1 decoy ODNs inhibited pro‑fibrotic gene expression in rat HSCS by downregulating TGF‑β signaling. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|