Novel dammarane saponins from Gynostemma pentaphyllum and their cytotoxic activities against HepG2 cells
| Autor: | Xiang-Lan Piao, Dao-Jin Chen, Cai-Xia Lou, Shao-Fang Xing |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Clinical Biochemistry
Pharmaceutical Science Biochemistry Terpene Structure-Activity Relationship chemistry.chemical_compound Drug Discovery Botany Humans Gynostemma pentaphyllum Molecular Biology IC50 Cell Proliferation Chromatography Dose-Response Relationship Drug Molecular Structure biology Organic Chemistry Dammarane Hep G2 Cells Saponins biology.organism_classification Antineoplastic Agents Phytogenic Triterpenes In vitro Gynostemma chemistry Molecular Medicine Drug Screening Assays Antitumor Two-dimensional nuclear magnetic resonance spectroscopy Heteronuclear single quantum coherence spectroscopy |
| Zdroj: | Bioorganic & Medicinal Chemistry Letters. 24:4831-4833 |
| ISSN: | 0960-894X |
| DOI: | 10.1016/j.bmcl.2014.08.059 |
| Popis: | Two new dammarane saponins, 2α,3β,12β-trihydroxydammar-20(22),24-diene-3-O-[β-D-glucopyranoxyl(1→2)-β-D-6″-O-acetylglucopyranoside (1, namely damulin C) and 2α,3β,12β-trihydroxydammar-20(21),24-diene-3-O-[β-D-glucopyranoxyl(1→2)-β-D-6″-O-acetylglucopyranoside (2, namely damulin D), were isolated from the ethanol extract of Gynostemma pentaphyllum, which had been heat processed by steaming at 125 °C. The NMR spectroscopic data of the novel saponins were completely assigned by using a combination of 2D NMR experiments including (1)H-(1)H COSY, HSQC, and HMBC. Their cytotoxic activities of human liver adenocarcinoma HepG2 cells were evaluated in vitro. They showed cytotoxicities against HepG2 cell line with IC50 of 40±0.7 and 38±0.5 μg/ml, respectively. |
| Databáze: | OpenAIRE |
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