Study of Receptor-Chaperone Interactions Using the Optical Technique of Spectroscopic Ellipsometry
| Autor: | David Smith, Mohd Kamarulzaki Mustafa, Alexei Nabok, Joanne Lacey, A. Tsargorodskaya, Tatiana M. Vinogradova, Benjamin Abell, Verena Kriechbaumer |
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| Rok vydání: | 2011 |
| Předmět: |
0106 biological sciences
Gene isoform Optics and Photonics Kinetics Arabidopsis Spectroscopy Imaging and Other Techniques Biophysics Receptors Cell Surface Plasma protein binding Models Biological 01 natural sciences 03 medical and health sciences Ellipsometry Animals HSP70 Heat-Shock Proteins HSP90 Heat-Shock Proteins 030304 developmental biology 0303 health sciences biology Arabidopsis Proteins Chemistry Spectrum Analysis Hsp90 Transport protein Protein Transport Crystallography Chaperone (protein) Calibration biology.protein Chaperone binding Rabbits Molecular Chaperones Protein Binding 010606 plant biology & botany |
| Zdroj: | Biophysical Journal. 101:504-511 |
| ISSN: | 0006-3495 |
| Popis: | This work describes a detailed quantitative interaction study between the novel plastidial chaperone receptor OEP61 and isoforms of the chaperone types Hsp70 and Hsp90 using the optical method of total internal reflection ellipsometry (TIRE). The receptor OEP61 was electrostatically immobilized on a gold surface via an intermediate layer of polycations. The TIRE measurements allowed the evaluation of thickness changes in the adsorbed molecular layers as a result of chaperone binding to receptor proteins. Hsp70 chaperone isoforms but not Hsp90 were shown to be capable of binding OEP61. Dynamic TIRE measurements were carried out to evaluate the affinity constants of the above reactions and resulted in clear discrimination between specific and nonspecific binding of chaperones as well as differences in binding properties between the highly similar Hsp70 isoforms. |
| Databáze: | OpenAIRE |
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