Overexpression of geranyl diphosphate synthase (PmGPPS1) boosts monoterpene and diterpene production involved in the response to pine wood nematode invasion
| Autor: | Yini Xie, Qinghua Liu, Bin Liu, Zhichun Zhou, Hengfu Yin |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Nematoda
biology Physiology Transgene Nicotiana benthamiana Bursaphelenchus xylophilus Plant Science Mevalonic acid Pinus biology.organism_classification Terpenoid Diphosphates Metabolic engineering Terpene chemistry.chemical_compound Biochemistry chemistry Monoterpenes Animals Diterpenes Gene Plant Diseases |
| Zdroj: | Tree Physiology. 42:411-424 |
| ISSN: | 1758-4469 |
| DOI: | 10.1093/treephys/tpab103 |
| Popis: | Outbreaks of pine wood nematode (PWN; Bursaphelenchus xylophilus) represent a severe biotic epidemic for the Pinus massoniana in China. When invaded by the PWN, the resistant P. massoniana might secret abundant oleoresin terpenoid to form certain defensive fronts for survival. However, the regulatory mechanisms of this process remain unclear. Here, the geranyl diphosphate synthase (PmGPPS1) gene was identified from resistant P. massoniana. Tissue-specific expression patterns of PmGPPS1 at transcript and protein level in resistant P. massoniana were determined by quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry. Functional characteristics analysis of PmGPPS1 was performed on transgenic Nicotiana benthamiana by overexpression, as genetic transformation of P. massoniana is, so far, not possible. In summary, we identified and functionally characterized PmGPPS1 from the resistant P. massoniana following PWN inoculation. Tissue-specific expression patterns and localization of PmGPPS1 indicated that it may play a positive role involved in the metabolic and defensive processes of oleoresin terpenes production in response to PWN attack. Furthermore, overexpression of PmGPPS1 may enhance the production of monoterpene, among which limonene reduced the survival of PWN in vitro. In addition, PmGPPS1 upregulated the expression level of key genes involved in mevalonic acid (MVA) pathway, the methylerythritol phosphate (MEP) pathway and gibberellins (GAs) biosynthesis to boost the growth and development of tobacco through a feedback regulation mechanism. Our results offered new insights into the pivotal role of the PmGPPS1 involved in terpene-based defense mechanisms responding to the PWN invasion in resistant P. massoniana and provided a new metabolic engineering scenario to improve monoterpene production in tobacco. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|