Effects of cigarette smoke on the antibody responses to thymic independent antigens from different lymphoid tissues of mice
| Autor: | Alan M. Kaplan, S N Goud, Bondada Subbarao |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Lymphoid Tissue
Health Toxicology and Mutagenesis Bone Marrow Cells Hemolytic Plaque Technique chemical and pharmacologic phenomena Spleen Toxicology Andrology Mice Immune system Antigen Bone Marrow Smoke medicine Animals Mesenteric lymph nodes Lymph node B-Lymphocytes business.industry Smoking Povidone General Medicine Antigens T-Independent Mice Inbred C57BL medicine.anatomical_structure Lymphatic system Mice Inbred DBA Antibody Formation Immunology Toxicity Female Lymph Nodes Bone marrow business |
| Zdroj: | Archives of Toxicology. 66:164-169 |
| ISSN: | 1432-0738 0340-5761 |
| DOI: | 10.1007/bf01974009 |
| Popis: | The influence of cigarette smoke on the humoral immune response of mice was investigated in lymphocytes derived from the spleen, bone marrow (BM) and mesenteric lymph nodes (MLN). Mice of the DBA/2J or C57BL/6 strain were exposed to cigarette smoke of a standard research cigarette, 2R1, twice a day, ten puffs each in morning and afternoon for 20, 40 or 60 weeks. At the end of the smoking period, animals were immunized intraperitoneally with the thymic independent antigens polyvinyl pyrrolidone (PVP) or trinitrophenyl (TNP)-Ficoll. The antibody responses were analyzed using sheep red blood cells coated with PVP or TNP, in a plaque forming cell (PFC) assay. The results indicate a statistically significant inhibition of the antibody response induced by PVP but not by TNP-Ficoll in splenic B cells of smoke exposed mice compared to sham controls. When tested in other lymphoid organs, there was higher anti-TNP PFC response from the BM and MLN cells of smoke exposed animals compared to sham controls. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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