Generation of induced pluripotent stem cell line (IPTi002-A) from an 87-year old sporadic Alzheimer's disease patient with APOE3 (ε3/ε3) genotype
| Autor: | Kang Xiahou, Xiaohui Liu, Yongxiang Zhang, Wenxia Zhou, Qiao Ren, Gang Liu, Shulin Meng, Ning Jiang, Lin Zhang |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Homeobox protein NANOG Genotype QH301-705.5 Cellular differentiation Induced Pluripotent Stem Cells Apolipoprotein E3 Embryoid body Biology Peripheral blood mononuclear cell Flow cytometry 03 medical and health sciences Kruppel-Like Factor 4 0302 clinical medicine SOX2 Alzheimer Disease medicine Humans Biology (General) Induced pluripotent stem cell Aged 80 and over medicine.diagnostic_test Cell Differentiation Cell Biology General Medicine 030104 developmental biology KLF4 embryonic structures Cancer research Leukocytes Mononuclear Female biological phenomena cell phenomena and immunity 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Stem Cell Research, Vol 53, Iss, Pp 102282-(2021) |
| ISSN: | 1876-7753 |
| Popis: | Here, we described the generation of human induced pluripotent stem cells (iPSC) from peripheral blood mononuclear cells (PBMCs) of a 87-year-old female patient with sporadic Alzheimer's disease (sAD) having APOE3 (ε3/ε3) genotype. iPSC line were generated from PBMCs with four factors of OCT4, SOX2, c-MYC and KLF4 using episomal system. The pluripotency of the iPSC line was assessed by embryoid body (EB) formation. Flow cytometry analyses revealed >97% cells positive for the pluripotency markers NANOG, OCT4 and SSEA4. Furthermore, the iPSC line displayed a normal karyotype (46, XX). The iPSC line may provide valuable tools for the study of sAD pathogenesis. |
| Databáze: | OpenAIRE |
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