The effect of syndecan-4 and glypican-1 expression on age-related changes in myogenic satellite cell proliferation, differentiation, and fibroblast growth factor 2 responsiveness
| Autor: | Sandra G. Velleman, Douglas C. McFarland, Laura B. Harthan |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Male
Turkeys animal structures Satellite Cells Skeletal Muscle Physiology Cell Biology Fibroblast growth factor MyoD Biochemistry Syndecan 1 Glypicans medicine Animals Molecular Biology Myogenin Cells Cultured Cellular Senescence Cell Proliferation Cell growth Cell Differentiation Molecular biology Cell biology carbohydrates (lipids) medicine.anatomical_structure embryonic structures Myogenic regulatory factors Fibroblast Growth Factor 2 Syndecan-4 Stem cell |
| Zdroj: | Comparative biochemistry and physiology. Part A, Molecularintegrative physiology. 166(4) |
| ISSN: | 1531-4332 |
| Popis: | Satellite cells are multipotential stem cells responsible for muscle growth and regeneration. Satellite cell proliferation, differentiation, and responsiveness to fibroblast growth factor 2 (FGF2) is, in part, regulated by the heparan sulfate proteoglycans syndecan-4 and glypican-1. Syndecan-4 and glypican-1 expression declines with satellite cell age and may be associated with decreased satellite cell activity. The objective of the current study was to determine if overexpression of syndecan-4 and glypican-1 would increase proliferation, differentiation and FGF2 responsiveness in satellite cells isolated from pectoralis major muscle from 16-wk-old turkeys. Overexpression of syndecan-4 and glypican-1 did not have a significant effect on proliferation and differentiation in 1 d, 7 wk, and 16 wk satellite cells, and did not affect FGF2 responsiveness during proliferation. Expression of syndecan-4 and glypican-1 increased differentiation at 48 h in 1 d, 7 wk, and 16 wk cells treated with FGF2. Expression of myogenic regulatory factors MyoD, myogenin, and MRF4 was affected by the overexpression of syndecan-4 and glypican-1. However, changes in myogenic regulatory factor expression did not have a significant effect on proliferation or differentiation. These data demonstrate that syndecan-4 and glypican-1 are likely not directly associated with the age related decrease in satellite cell activity. |
| Databáze: | OpenAIRE |
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