Identification of Escherichia coli DNA helicase IV with the use of a DNA helicase activity gel
| Autor: | David McCarthy, V N Trieu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1989 |
| Předmět: |
biology
DNA polymerase Circular bacterial chromosome Molecular Sequence Data DNA Helicases Helicase Microbiology RNA Helicase A Molecular biology Molecular Weight chemistry.chemical_compound Biochemistry chemistry Prokaryotic DNA replication biology.protein Escherichia coli Replisome Biological Assay Electrophoresis Polyacrylamide Gel Primase Amino Acid Sequence Molecular Biology DNA Research Article |
| Popis: | A DNA helicase activity gel was developed based on the assumption that DNA helicases could unwind double-stranded DNA in a polyacrylamide matrix. The production of single-stranded DNA was detected by staining the activity gel with acridine orange and visualizing the gel under long-wave UV light. The products of DNA helicase activities appeared as red bands within a green fluorescent background. A novel DNA helicase, called helicase IV, was detected in crude extracts of Escherichia coli with the use of the helicases activity gel assay. The new DNA helicase was purified to near homogeneity. The chromatographic properties and the sequence of its 11 amino-terminal residues proved that helicase IV was distinct from all of the previously described DNA helicases from E. coli. |
| Databáze: | OpenAIRE |
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