Nanodiamond–Gadolinium(III) Aggregates for Tracking Cancer Growth In Vivo at High Field
| Autor: | Emily A. Waters, Daniel J. Mastarone, Thomas J. Meade, Nikhil Rammohan, Laura M. Lilley, Adam T. Preslar, Giacomo Parigi, Laura K. Moore, Claudio Luchinat, Lisa M. Manus, Keith W. MacRenaris, Abigail Filicko, Dean Ho |
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| Rok vydání: | 2016 |
| Předmět: |
Materials science
Biocompatibility Gadolinium Contrast Media chemistry.chemical_element Bioengineering Mice SCID 02 engineering and technology 010402 general chemistry 01 natural sciences Article Nanodiamonds Ionizing radiation Mice Nuclear magnetic resonance In vivo medicine Animals Distribution (pharmacology) General Materials Science medicine.diagnostic_test Mechanical Engineering Cancer Magnetic resonance imaging Neoplasms Experimental General Chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics medicine.disease Magnetic Resonance Imaging Molecular Imaging 0104 chemical sciences cancer gadolinium in vivo MRI Chemistry (all) Materials Science (all) chemistry Cancer cell Female 0210 nano-technology |
| Zdroj: | Nano Letters. 16:7551-7564 |
| ISSN: | 1530-6992 1530-6984 |
| DOI: | 10.1021/acs.nanolett.6b03378 |
| Popis: | The ability to track labeled cancer cells in vivo would allow researchers to study their distribution, growth, and metastatic potential within the intact organism. Magnetic resonance (MR) imaging is invaluable for tracking cancer cells in vivo as it benefits from high spatial resolution and the absence of ionizing radiation. However, many MR contrast agents (CAs) required to label cells either do not significantly accumulate in cells or are not biologically compatible for translational studies. We have developed carbon-based nanodiamond–gadolinium(III) aggregates (NDG) for MR imaging that demonstrated remarkable properties for cell tracking in vivo. First, NDG had high relaxivity independent of field strength, a finding unprecedented for gadolinium(III) [Gd(III)]–nanoparticle conjugates. Second, NDG demonstrated a 300-fold increase in the cellular delivery of Gd(III) compared to that of clinical Gd(III) chelates without sacrificing biocompatibility. Further, we were able to monitor the tumor growth of NDG-labeled flank tumors by T1- and T2-weighted MR imaging for 26 days in vivo, longer than was reported for other MR CAs or nuclear agents. Finally, by utilizing quantitative maps of relaxation times, we were able to describe tumor morphology and heterogeneity (corroborated by histological analysis), which would not be possible with competing molecular imaging modalities. |
| Databáze: | OpenAIRE |
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