Functional analyses of small secreted cysteine‐rich proteins identified candidate effectors in Verticillium dahliae

Autor: Jie Wang, Dan-Dan Zhang, Sabiu Adamu, Jie-Yin Chen, Li Tian, Dan Wang, Xiaofeng Dai, Steven J. Klosterman, Lei Zhou, Zhi-Qiang Kong, Jun-Jiao Li, Krishna V. Subbarao, Shuang-Shuang Song, Chun‐Mei Yin, Ting-Gang Li, Yan Liu, Bao‐Li Wang, Jian Song
Rok vydání: 2020
Předmět:
0106 biological sciences
0301 basic medicine
Candidate gene
pathogen‐associated molecular pattern (PAMPs)
Plant Biology
Nicotiana benthamiana
Plant Science
01 natural sciences
chemistry.chemical_compound
Gene Expression Regulation
Plant
2.1 Biological and endogenous factors
Aetiology
Virulence
biology
Effector
small cysteine-rich proteins
food and beverages
pathogen-associated molecular pattern
Cell biology
Infectious Diseases
effector
Host-Pathogen Interactions
Original Article
Infection
small cysteine‐rich proteins (SCPs)
Crop and Pasture Production
Plant Biology & Botany
Soil Science
Mutagenesis (molecular biology technique)
Microbiology
03 medical and health sciences
Ascomycota
Genetics
Verticillium dahliae
Molecular Biology
Gene
disulphide bonds
Plant Diseases
Pathogen-Associated Molecular Pattern Molecules
fungi
Callose
Plant
Original Articles
biology.organism_classification
immunity
030104 developmental biology
Gene Expression Regulation
chemistry
Agronomy and Crop Science
010606 plant biology & botany
Zdroj: Molecular Plant Pathology
Molecular plant pathology, vol 21, iss 5
ISSN: 1364-3703
1464-6722
DOI: 10.1111/mpp.12921
Popis: Secreted small cysteine‐rich proteins (SCPs) play a critical role in modulating host immunity in plant–pathogen interactions. Bioinformatic analyses showed that the fungal pathogen Verticillium dahliae encodes more than 100 VdSCPs, but their roles in host–pathogen interactions have not been fully characterized. Transient expression of 123 VdSCP‐encoding genes in Nicotiana benthamiana identified three candidate genes involved in host–pathogen interactions. The expression of these three proteins, VdSCP27, VdSCP113, and VdSCP126, in N. benthamiana resulted in cell death accompanied by a reactive oxygen species burst, callose deposition, and induction of defence genes. The three VdSCPs mainly localized to the periphery of the cell. BAK1 and SOBIR1 (associated with receptor‐like protein) were required for the immunity triggered by these three VdSCPs in N. benthamiana. Site‐directed mutagenesis showed that cysteine residues that form disulphide bonds are essential for the functioning of VdSCP126, but not VdSCP27 and VdSCP113. VdSCP27, VdSCP113, and VdSCP126 individually are not essential for V. dahliae infection of N. benthamiana and Gossypium hirsutum, although there was a significant reduction of virulence on N. benthamiana and G. hirsutum when inoculated with the VdSCP27/VdSCP126 double deletion strain. These results illustrate that the SCPs play a critical role in the V. dahliae–plant interaction via an intrinsic virulence function and suppress immunity following infection.
Small cysteine‐rich proteins secreted by Verticillium dahliae play critical roles in interactions with hosts via an intrinsic virulence function and can suppress immunity following infection.
Databáze: OpenAIRE
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