Influence of Gelatin-Thrombin Matrix Tissue Sealant on Bacterial Colony Formation and Risk of Pelvic Infection

Autor: Bruce D. McCollister, Daniel N. Frank, Andrés Vázquez-Torres, Patrick K. Henthorn, Kian Behbakht, Michael Jarrett, Hiba Q. Anwar, Diana Ir, Jeanelle Sheeder, Michael S. Guy
Rok vydání: 2015
Předmět:
Pathology
medicine.medical_treatment
Colony Count
Microbial
030204 cardiovascular system & hematology
medicine.disease_cause
0302 clinical medicine
Postoperative Complications
Risk Factors
Medicine
Saline
Aged
80 and over
030219 obstetrics & reproductive medicine
biology
Pelvic Infection
Thrombin
Obstetrics and Gynecology
Middle Aged
Infectious Diseases
medicine.anatomical_structure
Vagina
Female
Research Article
Adult
medicine.medical_specialty
Article Subject
Adolescent
Dermatology
Hysterectomy
lcsh:Gynecology and obstetrics
Enterococcus faecalis
lcsh:Infectious and parasitic diseases
Microbiology
03 medical and health sciences
Young Adult
Escherichia coli
Humans
lcsh:RC109-216
lcsh:RG1-991
Aged
business.industry
Bacteria Present
biology.organism_classification
Hemostasis
Surgical
Gelatin
Tissue Adhesives
business
Bacteria
Zdroj: Infectious Diseases in Obstetrics and Gynecology
Infectious Diseases in Obstetrics and Gynecology, Vol 2016 (2016)
ISSN: 1098-0997
Popis: Objective. Gelatin-thrombin matrix (GTM) tissue sealant use was previously identified as an independent predictor of pelvic infection following hysterectomies. We aim to elucidate contributing factors by assessing influence of GTM on bacterial colony formation and characterizing bacteria present at the vaginal cuff.Methods.Escherichia coliwas incubated in phosphate-buffered saline (PBS) and pelvic washings with and without GTM to assess influence on colony formation. Pelvic washings of the vaginal cuff were collected from hysterectomies occurring from June through October 2015.In vitrotechniques, 16S rRNA gene qPCR, and 16S amplicon sequencing were performed with washings to characterize bacteria at the vaginal cuff.Results. Mean bacterial colony formation in PBS was greater forE. coliincubated in the presence of GTM (1.48 × 107 CFU/mL) versus without (9.95 × 105 CFU/mL) following 20-hour incubation (p=0.001). Out of 61 pelvic washings samples, 3 were culture positive (≥5000 CFU/mL) withEnterococcus faecalis.Conclusion.In vitroexperiments support a facilitating role of GTM on colony formation ofE. coliin PBS. However, given the negative results of surgical site washings following adequate disinfection, the role of GTM in promoting posthysterectomy pelvic infections may be limited. Analysis of pelvic washings revealed presence ofE. faecalis, but results were inconclusive. Further studies are recommended.
Databáze: OpenAIRE
Externí odkaz: