Structure of Pigment Metabolic Pathways and Their Contributions to White Tepal Color Formation of Chinese Narcissus tazetta var. chinensis cv Jinzhanyintai
| Autor: | Yujun Ren, Yaping Jiang, Jingwen Yang, Haiyang Chen, Bingguo Lu, Ying Miao, Binghua Wu, Yuwei Hong |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Phytochemicals 01 natural sciences metabolite analysis lcsh:Chemistry Transcriptome chemistry.chemical_compound Gene Expression Regulation Plant Chinese narcissus pigment metabolic pathway tepal pigmentation comparative KEGG pathway enrichment lcsh:QH301-705.5 Carotenoid Spectroscopy chemistry.chemical_classification biology Pigmentation food and beverages Narcissus General Medicine Computer Science Applications Tepal Phenotype visual_art visual_art.visual_art_medium Metabolic Networks and Pathways Flowers Article Catalysis Inorganic Chemistry 03 medical and health sciences Pigment Botany Physical and Theoretical Chemistry Narcissus tazetta Molecular Biology Gene Expression Profiling Organic Chemistry Computational Biology Reproducibility of Results Molecular Sequence Annotation Pigments Biological biology.organism_classification Metabolic pathway 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 chemistry Chlorophyll 010606 plant biology & botany |
| Zdroj: | International Journal of Molecular Sciences, Vol 18, Iss 9, p 1923 (2017) International Journal of Molecular Sciences; Volume 18; Issue 9; Pages: 1923 International Journal of Molecular Sciences |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms18091923 |
| Popis: | Chinese narcissus (Narcissus tazetta var. chinensis) is one of the ten traditional flowers in China and a famous bulb flower in the world flower market. However, only white color tepals are formed in mature flowers of the cultivated varieties, which constrains their applicable occasions. Unfortunately, for lack of genome information of narcissus species, the explanation of tepal color formation of Chinese narcissus is still not clear. Concerning no genome information, the application of transcriptome profile to dissect biological phenomena in plants was reported to be effective. As known, pigments are metabolites of related metabolic pathways, which dominantly decide flower color. In this study, transcriptome profile and pigment metabolite analysis methods were used in the most widely cultivated Chinese narcissus “Jinzhanyintai” to discover the structure of pigment metabolic pathways and their contributions to white tepal color formation during flower development and pigmentation processes. By using comparative KEGG pathway enrichment analysis, three pathways related to flavonoid, carotenoid and chlorophyll pigment metabolism showed significant variations. The structure of flavonoids metabolic pathway was depicted, but, due to the lack of F3ʹ5ʹH gene; the decreased expression of C4H, CHS and ANS genes; and the high expression of FLS gene, the effect of this pathway to synthesize functional anthocyanins in tepals was weak. Similarly, the expression of DXS, MCT and PSY genes in carotenoids synthesis sub-pathway was decreased, while CCD1/CCD4 genes in carotenoids degradation sub-pathway was increased; therefore, the effect of carotenoids metabolic pathway to synthesize adequate color pigments in tepals is restricted. Interestingly, genes in chlorophyll synthesis sub-pathway displayed uniform down-regulated expression, while genes in heme formation and chlorophyll breakdown sub-pathways displayed up-regulated expression, which also indicates negative regulation of chlorophyll formation. Further, content change trends of various color metabolites detected by HPLC in tepals are consistent with the additive gene expression patterns in each pathway. Therefore, all three pathways exhibit negative control of color pigments synthesis in tepals, finally resulting in the formation of white tepals. Interestingly, the content of chlorophyll was more than 10-fold higher than flavonoids and carotenoids metabolites, which indicates that chlorophyll metabolic pathway may play the major role in deciding tepal color formation of Chinese narcissus. |
| Databáze: | OpenAIRE |
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