| Autor: |
Rosane Silva, Tais Hanae Kasai-Brunswick, Claudio H. Gress, Rosalia Mendez-Otero, Pedro Hernan Cabello, Marli P.S. Loureiro, Isadora M. Vaz, Pablo Domizi, Tatiane D. Cozendey, Fernanda Gubert, Tamara Borgonovo, Juliana Ferreira Vasques, Giselda M. K. Cabello, José Mauro Braz de Lima, María Fernanda Toledo |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Předmět: |
|
| Zdroj: |
Stem Cell Research, Vol 37, Iss, Pp-(2019) |
| ISSN: |
1873-5061 |
| Popis: |
Induced pluripotent stem cell (iPSC) lines were generated from erythroblasts of two patients with amyotrophic lateral sclerosis (ALS) and two healthy individuals. One familial and one sporadic ALS patients were used, both with genetic alterations in VAPB gene. CytoTune™-iPS 2.0 Sendai Reprogramming Kit (containing the reprogramming factors OCT3/4, KLF4, SOX2 and cMYC) was used to generate the iPSC cell lines. The four iPSCs express pluripotency markers, have normal karyotype and differentiated spontaneously in the three germ layers. The expression of Sendai virus was lost in all iPSC lines after 15 passages. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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