Evidence for a membrane skeleton in higher plants A spectrin-like polypeptide co-isolates with rice root plasma membranes
| Autor: | Roger M. Spanswick, Christopher D. Faraday |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Azides
Erythrocytes Immunoblotting Biophysics Cell Fractionation Biochemistry Aqueous polymer two-phase partitioning Structural Biology Microsomes Genetics Humans Spectrin Bovine serum albumin Cytoskeleton Molecular Biology Adenosine Triphosphatases Molybdenum chemistry.chemical_classification Differential centrifugation Nitrates biology Chemistry Cell Membrane Peripheral membrane protein Oryza Cell Biology Hydrogen-Ion Concentration Plants Mitochondria Peripheral protein Fodrin Membrane Polyclonal antibodies biology.protein Electrophoresis Polyacrylamide Gel Vanadates Glycoprotein Cytoskeleton-plasma membrane interaction |
| Zdroj: | FEBS Letters. (3):313-316 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(93)80536-4 |
| Popis: | A fraction enriched in plasma membranes was isolated from rice roots by differential centrifugation and aqueous polymer two-phase partitioning. Analysis of the fraction by SDS-PAGE showed the presence of several low mobility polypeptides (M(r)100 kDa). One of these polypeptides (M(r) approximately 230 kDa) was specifically recognized by polyclonal antibodies to human erythrocyte spectrin. This finding suggests that a higher plant spectrin-based membrane skeleton may be preserved and studied using high-purity plasma membrane fractions obtained by aqueous polymer two-phase partitioning. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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