Detection of lysozyme in llama, sheep, and cattle tears
| Autor: | Sheryl G. Krohne, Juliet R. Gionfriddo, Elikplimi K. Asem, Harriet J. Davidson |
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| Rok vydání: | 2000 |
| Předmět: |
Protein band
Blotting Western Micrococcus Microbiology chemistry.chemical_compound Microtiter plate Western blot medicine Animals Eye Proteins Sheep General Veterinary medicine.diagnostic_test biology General Medicine biology.organism_classification eye diseases Tear lysozyme chemistry Tears Immunoassay Immunology Cattle Colorimetry Electrophoresis Polyacrylamide Gel Muramidase sense organs Lysozyme Camelids New World |
| Zdroj: | American Journal of Veterinary Research. 61:1294-1297 |
| ISSN: | 0002-9645 |
| Popis: | Objective—To determine whether the tears of llamas, sheep, and cattle contain lysozyme and compare lysozyme concentrations in tears among these species. Animals—40 llamas, 5 sheep, and 36 cattle. Procedure—Electrophoresis, western blot immunoassay for lysozyme, a spectrophotometric assay to detect tear lysozyme by its ability to lyse a suspension of Micrococcus lysodeiticus, and a microtiter plate colorometric assay were performed. Results—A 13.6-kd protein band was detected by use of electrophoresis and western blot immunoassay in llama and sheep tears but not cattle tears. Results of spectrophotometric assay suggested that llama and sheep tears had high concentrations of lysozyme, whereas cattle tears had low concentrations. Results of the microtiter plate colorometric assay suggested that llama tears had high concentrations of lysozyme, whereas concentrations in sheep and cattle tears were lower. Conclusions and Clinical Relevance—Lysozyme concentrations in tears may vary among species and this variability may contribute to differing susceptibilities to ocular diseases such as infectious keratoconjunctivitis. (Am J Vet Res 2000;61:1294–1297) |
| Databáze: | OpenAIRE |
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