Micro-ribonucleic acids and extracellular vesicles repertoire in the spent culture media is altered in women undergoing In Vitro Fertilization
Autor: | Masood Abu-Halima, Sigrun Nestel, Claudia Staib, Andreas Keller, Irina Nazarenko, Tobias Fehlmann, Eckart Meese, Sebastian Häusler, Christina Backes |
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Rok vydání: | 2017 |
Předmět: |
Adult
0301 basic medicine medicine.medical_treatment lcsh:Medicine Fertilization in Vitro Biology Real-Time Polymerase Chain Reaction Article Andrology Extracellular Vesicles 03 medical and health sciences Pregnancy microRNA medicine Humans Gene Regulatory Networks ddc:610 lcsh:Science Oligonucleotide Array Sequence Analysis Regulation of gene expression Multidisciplinary In vitro fertilisation Microarray analysis techniques lcsh:R fungi Embryo Embryo Transfer Embryo Mammalian medicine.disease Embryo transfer Culture Media MicroRNAs 030104 developmental biology Real-time polymerase chain reaction Immunology Female lcsh:Q |
Zdroj: | Scientific Reports, Vol 7, Iss 1, Pp 1-11 (2017) Scientific Reports |
ISSN: | 2045-2322 |
Popis: | MicroRNAs (miRNAs) are class of small RNA molecules with major impact on gene regulation. We analyzed the potential of miRNAs secreted from pre-implantation embryos into the embryonic culture media as biomarkers to predict successful pregnancy. Using microarray analysis, we profiled the miRNome of the 56 spent culture media (SCM) after embryos transfer and found a total of 621 miRNAs in the SCM. On average, we detected 163 miRNAs in SCM of samples with failed pregnancies, but only 149 SCM miRNAs of embryos leading to pregnancies. MiR-634 predicted an embryo transfer leading to a positive pregnancy with an accuracy of 71% and a sensitivity of 85%. Among the 621 miRNAs, 102 (16.4%) showed a differential expression between positive and negative outcome of pregnancy with miR-29c-3p as the most significantly differentially expressed miRNA. The number of extracellular vehicles was lower in SCM with positive outcomes (3.8 × 109/mL EVs), as compared to a negative outcome (7.35 × 109/mL EVs) possibly explaining the reduced number of miRNAs in the SCM associated with failed pregnancies. The analysis of the miRNome in the SCM of couples undergoing fertility treatment lays the ground towards development of biomarkers to predict successful pregnancy and towards understanding the role of embryonic miRNAs found in the SCM. |
Databáze: | OpenAIRE |
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