Leukapheresis cell concentration adjustment required for a successful recovery of HSC after cryopreservation
Autor: | Dereen Ahmad, Marco Possenti, Hanar A. Abdulrahman, Ignazio Majolino, Michele Vacca, Dastan O. Hassan, Dosti Othman, Rebar N. Mohammed, Harem Omer, Diana Faruq, Najmaddin Khoshnaw, Salah Muhamad |
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Rok vydání: | 2020 |
Předmět: |
Adult
Blood Platelets Male Cell Survival Neutrophils Antigens CD34 Cell Count Transplantation Autologous General Biochemistry Genetics and Molecular Biology Cryopreservation Flow cytometry Andrology 03 medical and health sciences 0302 clinical medicine medicine Humans Autologous transplantation Leukapheresis Bone Marrow Transplantation 030219 obstetrics & reproductive medicine medicine.diagnostic_test business.industry Hematopoietic Stem Cell Transplantation 0402 animal and dairy science 04 agricultural and veterinary sciences General Medicine Middle Aged Hematopoietic Stem Cells 040201 dairy & animal science Transplantation Haematopoiesis Apheresis Female Stem cell General Agricultural and Biological Sciences business |
Zdroj: | Cryobiology. 92:21-25 |
ISSN: | 0011-2240 |
Popis: | The recovering of an adequate number of hematopoietic stem cells after cryopreservation is considered pivotal for successful transplantation. Various factors could influence the recovery of HSC following processing and cryopreservation. Therefore, leukapheresis product from thirty patients was cryopreserved in 10% DMSO in cryopreservation bags for their autologous bone marrow transplantation, and 2 ml were cryopreserved in cryovials for post-thaw viability assessment by flow cytometry. The percentage of viable HSCs recovered post-cryopreservation in leukapheresis product was significantly influenced by the concentration of the total nucleated cells cryopreserved per volume. Patients receiving a higher rate of viable HSCs resulted in earlier engraftment of both neutrophils and platelets, so they have been discharged earlier from the hospital. Furthermore, Storage temperature and duration played a role in the recovery of these cells and for the support of the findings, age of the patient at the time of collection did not show any impact on the recovery of this HSC post-cryopreservation. In conclusion, various influencing factors must be taken into consideration during the cryopreservation of HSCs, especially for poor mobilizing patients with a low number of collected hematopoietic stem cells. |
Databáze: | OpenAIRE |
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