Chromatin structure of heat-shock-inducible genes and abscisic acid-inducible genes in wheat

Autor:	Steven Spiker, Deborah S. Loer
Rok vydání:	1992
Předmět:	Physiology food and beverages Cell Biology Plant Science General Medicine Biology Molecular biology Chromatin chemistry.chemical_compound Biochemistry chemistry Transcription (biology) Gene expression Genetics Gene family Deoxyribonuclease I Abscisic acid Gene Southern blot
Zdroj:	Physiologia Plantarum. 86:77-84
ISSN:	1399-3054 0031-9317
DOI:	10.1034/j.1399-3054.1992.860111.x
Popis:	We have asked if the induction of gene expression by heat shock or by the phytohormone abscisic acid is accompanied by a change in chromatin structure as assayed by general DNase I sensitivity of the coding region of the responsive genes in wheat (Triticum aestivum L. cv. Stephens). Using nuclei isolated from wheat seedlings, we show by Southern blot hybridization that members of the WHSP 17 gene family (encoding a low-molecular-weight heat-shock protein) become more sensitive to DNase I upon heat shock. This result indicates that the induction of heat-shock genes is accompanied by a change in chromatin conformation in plants, as has been previously demonstrated in animal systems. Abscisic acid has previously been shown to induce the transcription of a 7S globulin gene and inhibit the expression of ribulose 1,5-bisphate carboxylase/oxygenase small subunit genes in wheat seedlings. We found both of these genes to be sensitive to DNase I before abscisic acid treatment, and we found no change upon treatment.
Databáze:	OpenAIRE
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