Screening ofBothrops snake venoms forl-amino acid oxidase activity
| Autor: | José D. Fontana, Maria F. D. Furtado, Lorenzo R. S. Zanette, Walliana T. Costa, Madalena Baron, Manoel F. Guimaraes, Marcos L. Pessatti |
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| Rok vydání: | 1995 |
| Předmět: |
Hemorrhage
Bioengineering Venom L-Amino Acid Oxidase L-amino-acid oxidase complex mixtures Applied Microbiology and Biotechnology Biochemistry Cofactor chemistry.chemical_compound Animals Bothrops Amino Acids Rats Wistar Molecular Biology Chromatography High Pressure Liquid Flavin adenine dinucleotide chemistry.chemical_classification Dose-Response Relationship Drug biology Spectrum Analysis Proteins General Medicine biology.organism_classification Molecular biology Rats Amino acid Zinc chemistry Snake venom Flavin-Adenine Dinucleotide biology.protein L-amino-acid oxidase activity Amino Acid Oxidoreductases Isoelectric Focusing Snake Venoms Biotechnology |
| Zdroj: | Applied Biochemistry and Biotechnology. :197-210 |
| ISSN: | 1559-0291 0273-2289 |
| DOI: | 10.1007/bf02933424 |
| Popis: | Toxins, enzymes, and biologically active peptides are the main components of snake venoms from the genus Bothrops. Following the venom inoculation, the local effects are hemorrhage, edema, and myonecrosis. Nineteen different species of Brazilian Bothrops were screened for protein content and L-amino acid oxidase activity. B. cotiara, formerly found in the South of Brazil, is now threatened with extinction. Its venom contains a highly hemorrhagic fraction and, as expected from the deep yellow color of the corresponding lyophilized powder, a high L-amino acid oxidase (LAO) activity was also characterized. Flavin adenine dinucleotide (FAD) is its associate coenzyme. B. cotiara venom LAO catalyzed the oxidative deamination of several L-amino acids, and the best substrates were methionine, leucine, tryptophan, and phenylalanine, hence, its potential application for the use of biosensors for aspartame determination and for the removal of amino acids from plasma. High levels for LAO were also found in other species than B. cotiara. In addition, the technique of isoelectric focusing (IEF) was employed as a powerful tool to study the iso- or multi-enzyme distribution for LAO activity in the B. cotiara snake venom. |
| Databáze: | OpenAIRE |
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