Size-Dependent Biodistribution of Fluorescent Furano-Allocolchicinoid-Chitosan Formulations in Mice
| Autor: | Alexey Yu. Fedorov, Dmitrii Aronov, E. V. Moiseeva, Elena V. Svirshchevskaya, Maria Konovalova, Iuliia A. Gracheva |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Biodistribution
Polymers and Plastics Organic chemistry Spleen 02 engineering and technology Article law.invention Chitosan 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine QD241-441 In vivo Confocal microscopy law medicine Rhodamine B Colchicine furano-allocolchicinoid biodistribution Wnt-1 breast tumor Chromatography technology industry and agriculture General Chemistry 021001 nanoscience & nanotechnology In vitro medicine.anatomical_structure chemistry 030220 oncology & carcinogenesis nano/micro particles chitosan 0210 nano-technology |
| Zdroj: | Polymers, Vol 13, Iss 2045, p 2045 (2021) Polymers Volume 13 Issue 13 |
| ISSN: | 2073-4360 |
| Popis: | The aim of this study was to compare the biodistribution in mice of functionalized rhodamine B (Rh) labeled colchicine derivative furano-allocolchicinoid (AC, 6) either conjugated to 40 kDa chitosan (AC-Chi, 8) or encapsulated into chitosan nanoparticles (AC-NPs). AC-NPs were formed by ionotropic gelation and were 400–450 nm in diameter as estimated in mice by dynamic light scattering and confocal microscopy. AC-Chi and AC-NPs preserved the specific colchicine activity in vitro. AC preparations were once IV injected into C75BL/6 mice muscles, spleen, kidney, liver, lungs, blood cells and serum were collected at 30 min, 2, 5, 10, and 20 h post injection. To analyze the distribution of the furano-allocolchicinoid preparations in body liquids and tissues, Rh was measured directly in sera or extracted by acidic ethanol from tissue homogenates. Preliminary Rh extraction rate was estimated in vitro in tissue homogenates and was around 25–30% from total quantity added. After in vivo injection, AC-NPs were accumulated more in liver and spleen, while less in kidney and lungs in comparison with free AC and AC-Chi. Therefore, incorporation of colchicine derivatives as well as other hydrophobic substances into nano/micro sized carriers may help redistribute the drug to different organs and, possibly, improve antitumor accumulation. |
| Databáze: | OpenAIRE |
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