Enhanced DNA repair by DNA photolyase bearing an artificial light-harvesting chromophore
| Autor: | Ryuma Sato, Risa Matsumura, Shigenori Iwai, Yuma Terai, Junpei Yamamoto |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
DNA Repair
Light DNA damage DNA repair AcademicSubjects/SCI00010 Biology Protein Engineering 03 medical and health sciences chemistry.chemical_compound Chemical Biology and Nucleic Acid Chemistry Genetics Photolyase Narese/6 030304 developmental biology Flavin adenine dinucleotide 0303 health sciences Thermus thermophilus 030302 biochemistry & molecular biology Photoreceptor protein DNA photolyase Chromophore Narese/20 Recombinant Proteins chemistry Biophysics Flavin-Adenine Dinucleotide Deoxyribodipyrimidine Photo-Lyase DNA DNA Damage |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 0305-1048 |
| Popis: | Photolyases are flavoenzymes responsible for the repair of carcinogenic DNA damage caused by ultraviolet radiation. They harbor the catalytic cofactor flavin adenine dinucleotide (FAD). The light-driven electron transfer from the excited state of the fully-reduced form of FAD to the DNA lesions causes rearrangement of the covalent bonds, leading to the restoration of intact nucleobases. In addition to the catalytic chromophore, some photolyases bear a secondary chromophore with better light absorption capability than FAD, acting as a light-harvesting chromophore that harvests photons in sunlight efficiently and transfers light energy to the catalytic center, as observed in natural photoreceptor proteins. Inspired by nature, we covalently and site-specifically attached a synthetic chromophore to the surface of photolyase using oligonucleotides containing a modified nucleoside and a cyclobutane-type DNA lesion, and successfully enhanced its enzymatic activity in the light-driven DNA repair. Peptide mapping in combination with theoretical calculations identified the amino acid residue that binds to the chromophore, working as an artificial light-harvesting chromophore. Our results broaden the strategies for protein engineering and provide a guideline for tuning of the light perception abilities and enzymatic activity of the photoreceptor proteins. |
| Databáze: | OpenAIRE |
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