Nitric oxide production by macrophages of dogs vaccinated with killed Leishmania infantum promastigotes
| Autor: | Vincenzo Mitolo, Antonio Fasanella, Margherita Sisto, Olga Brandonisio, F. Bertani, Sabrina Lisi, B. Consenti, Maria Antonietta Panaro, Teresa Trotta, Dario Domenico Lofrumento, A. Acquafredda |
|---|---|
| Přispěvatelé: | M. A., Panaro, A., Acquafredda, S., Lisi, Lofrumento, Dario Domenico, V., Mitolo, M., Sisto, A., Fasanella, T., Trotta, F., Bertani, B., Consenti, O., Brandonisio |
| Rok vydání: | 2001 |
| Předmět: |
Cytotoxicity
Immunologic Male Protozoan Vaccines Disease reservoir Phagocytosis Immunology ifn-g Nitric Oxide Microbiology Peripheral blood mononuclear cell NO Interferon-gamma Dogs medicine Concanavalin A Immunology and Allergy Macrophage Animals Humans Interferon gamma Dog Diseases Leishmania infantum Disease Reservoirs General Veterinary biology Macrophages Vaccination Leishmaniasis General Medicine Macrophage Activation medicine.disease biology.organism_classification Leishmania Infectious Diseases Italy macropage Leishmaniasis Visceral Female medicine.drug |
| Zdroj: | Comparative immunology, microbiology and infectious diseases. 24(3) |
| ISSN: | 0147-9571 |
| Popis: | Human visceral leishmaniosis is endemic in Southern Italy, where the dog is the main reservoir of viscerotropic strains of Leishmania infantum. The release of nitric oxide (NO) by interferon (IFN)-gamma-activated macrophages is an important leishmanicidal mechanism in several animal species. In this work NO production, phagocytosis and killing capacity of monocyte-derived dog macrophages were evaluated in vitro before and after administration of a vaccine composed of killed Leishmania infantum promastigotes. Moreover, IFN-gamma content was measured in concanavalin A-activated dog peripheral blood mononuclear cell (PBMC) supernatants employed for macrophage stimulation. Phagocytosis, killing capacity and NO production by canine macrophages increased significantly 1 month after vaccine administration, and the increase also persisted 5 months later. In addition, the amount of IFN-gamma in PBMC supernatants was significantly higher after vaccination. Overall, our results suggest the usefulness of evaluating the in vivo protective role of this promastigote preparation in dogs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect