Sea urchin embryos exposed to cadmium as an experimental model for studying the relationship between autophagy and apoptosis
Autor: | Maria Agnello, Liana Bosco, Maria Carmela Roccheri, Roberto Chiarelli |
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Přispěvatelé: | Chiarelli, R, Agnello, M, Bosco, L, Roccheri, MC |
Rok vydání: | 2014 |
Předmět: |
Programmed cell death
Embryo Nonmammalian Immunocytochemistry Apoptosis Aquatic Science Biology Oceanography Apoptosis Autophagy Stress Cadmium Sea urchin Embryo Paracentrotus lividus Autophagy Animals Settore BIO/06 - Anatomia Comparata E Citologia TUNEL assay Caspase 3 Embryo General Medicine biology.organism_classification Pollution Embryonic stem cell Cell biology Models Animal Paracentrotus Water Pollutants Chemical Cadmium |
Zdroj: | Marine Environmental Research. 93:47-55 |
ISSN: | 0141-1136 |
Popis: | The sea urchin embryo is a suitable model that offers an excellent opportunity to investigate different defence strategies activated in stress conditions. We previously showed that cadmium accumulates in a dose- and time-dependent manner into embryonic cells, activating different stress and defence mechanisms, including the synthesis of HSPs and the onset of apoptosis and/or autophagy. In this paper we investigated the functional relationship between autophagy and apoptosis, evaluating apoptosis signals in cadmium-exposed Paracentrotus lividus embryos with inhibited autophagy. We found that the inhibition of autophagy produced the concurrent reduction of apoptosis, suggesting that the two phenomena are functionally related. Considering the catabolic role of autophagy, an energetic hypothesis to explain the relationship was evaluated. Using a substrate for ATP production, we found that apoptosis, assessed by TUNEL and cleaved caspase-3 immunocytochemistry, was substantially restored in cadmium-treated embryos where autophagy was inhibited by 3-Methyladenine. On the basis of these results, we propose that, autophagy could play a crucial role in stress response of this organism because autophagy could energetically contribute to apoptotic execution through its catabolic role. |
Databáze: | OpenAIRE |
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