Comprehensive analysis of HIV Gag-specific IFN-γ response in HIV-1- and HIV-2-infected asymptomatic patients from a clinical cohort in The Gambia
| Autor: | Beatrice Ondondo, Lucy Dorrell, Kevin Peterson, Assan Jaye, Hilton Whittle, Matthew Cotten, Sarah Rowland-Jones |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Adult
Male Molecular Sequence Data Immunology Gene Products gag HIV Infections Immunodominance Cross Reactions Asymptomatic Epitope Cohort Studies Interferon-gamma Immune system Immunity medicine Humans Immunology and Allergy Amino Acid Sequence Cells Cultured Aged business.industry ELISPOT virus diseases Middle Aged Virology Viral replication HIV-2 HIV-1 Female Gambia medicine.symptom Peptides business Viral load |
| Zdroj: | European Journal of Immunology. 38:3549-3560 |
| ISSN: | 0014-2980 |
| Popis: | Majority of HIV-2-infected individuals meet the criteria of long-term non-progressors. This has been linked to superior qualitative HIV-2-specific cellular immune responses that correlate with viral control. However, it is unknown whether this is due to frequent targeting of immunodominant Gag epitopes in HIV-2 than HIV-1 infection. We describe a comprehensive comparison of the magnitude, breadth and frequency of Gag responses and the degree of cross-recognition of frequently targeted, immunodominant Gag peptides in a cross-sectional study of asymptomatic HIV-1- and HIV-2-infected individuals. Fresh PBMC from 20 HIV-1- and 20 HIV-2-infected patients with similar CD4(+) T-cell counts (p=0.36) were stimulated with pools of HIV-1 and/or HIV-2 Gag peptides in an IFN-gamma ELISPOT assay. We found no difference in the cumulative magnitude of IFN-gamma responses (p=0.75) despite significantly lower plasma viral loads in HIV-2-infected people (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text