Characteristics of sensory innervation in synovium of rats within different knee osteoarthritis models and the correlation between synovial fibrosis and hyperalgesia

Autor: Li Zhang, Mingchao Li, Xiaochen Li, Taiyang Liao, Zhenyuan Ma, Runlin Xing, Peimin Wang, Jun Mao
Rok vydání: 2022
Předmět:
0301 basic medicine
Medicine (General)
Pathology
Science (General)
Osteoarthritis
CGRP
calcitonin gene-related peptide
Q1-390
0302 clinical medicine
Basic and Biological Science
Fibrosis
Multidisciplinary
Synovial Membrane
MWT
mechanical withdrawal threshold
Synovial fibrosis
Sensory innervation
TRPV1
transient receptor potential vanilloid type 1
DMM
destabilization of the medial meniscus
Osteoarthritis
Knee
VEGF
vascular endothelial growth factor
KOA
knee osteoarthritis
ECM
extracellular matrix
Animal models
medicine.anatomical_structure
Hyperalgesia
030220 oncology & carcinogenesis
Knee osteoarthritis
medicine.symptom
Medial meniscus
Type I collagen
medicine.medical_specialty
Anterior cruciate ligament
NGF
nerve growth factor
TRPV1
Pain
Calcitonin gene-related peptide
03 medical and health sciences
R5-920
TGF-β
transforming growth factor-β
ACLT
anterior cruciate ligament transection
medicine
Animals
ComputingMethodologies_COMPUTERGRAPHICS
TWT
thermal withdrawal threshold
business.industry
MIA
monoiodoacetate
medicine.disease
Rats
Disease Models
Animal
030104 developmental biology
CWT
cold withdrawal threshold
business
Zdroj: Journal of Advanced Research, Vol 35, Iss, Pp 141-151 (2022)
Journal of Advanced Research
ISSN: 2090-1232
DOI: 10.1016/j.jare.2021.06.007
Popis: Graphical abstract
Highlights • Synovial fibrosis was positively correlated with pain sensitivity in KOA rats. • Synovial fibrosis was most prominent in DMM group 14 days after modeling. • ACLT replaced DMM to be the most typical at 28 days after modeling. • Increased synovial sensory innervation followed the same trend as fibrosis. • ACLT is more applicable for KOA pain research.
Introduction Knee osteoarthritis (KOA) showed synovial fibrosis and hyperalgesia, although the correlation between the two is unclear. Besides, the specific changes of sensory innervation in animal models are still controversial, which makes it difficult to choose the modeling methods for KOA pain research. Objectives Study the characteristics of sensory innervation within three commonly used KOA rat models and the correlation between synovial fibrosis and hyperalgesia. Methods KOA models were induced by destabilization of medial meniscus (DMM), anterior cruciate ligament transection (ACLT), and monoiodoacetate (MIA), respectively. Mechanical, cold and thermal withdrawal threshold (MWT, CWT and TWT) were measured. The harvested tissues were used for pathological sections, immunofluorescence and quantitative analysis. Results KOA synovium showed more type I collagen deposition, increased expression of CD31, VEGF and TGF-β. These changes were most pronounced in surgical models, with DMM presenting the most prominent at Day 14 and ACLT at Day 28. Day 14, changes in mechanical hyperalgesia and cold hyperalgesia were most typical in DMM model and statistically different from MIA. There was a negative correlation between the percentage of type I collagen and MWT value (r = −0.88), as well as CWT value (r = −0.95). DMM synovium showed more axonal staining, upregulated CGRP, TRPV1, NGF and Netrin1 compared with MIA. Above changes were also observed at Day 28, but ACLT replaced DMM as the most typical. In DRG, only the levels of CGRP and NGF were different among KOA models at Day 14, and the highest in DMM, which was statistically different compared with MIA. Conclusions This study described the details of sensory innervation in different KOA model of rats, and the degree of synovial fibrosis was positively correlated with the pain sensitivity of KOA model rats. Additionally, surgical modeling especially ACLT method is more recommended for KOA pain research.
Databáze: OpenAIRE
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