Impact of a transposon insertion in phzF2 on the specialized metabolite production and interkingdom interactions of Pseudomonas aeruginosa
| Autor: | Wilna J. Moree, Pieter C. Dorrestein, Vanessa V. Phelan, Kit Pogliano, Suzanne M. Noble, Julieta Aguilar, Carlos A. Guerrero, Alexandra Koumoutsi, Dale S. Cornett |
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| Rok vydání: | 2014 |
| Předmět: |
Cystic Fibrosis
Metabolite Virulence Biology medicine.disease_cause Medical and Health Sciences Microbiology Mass spectrometry imaging chemistry.chemical_compound Metabolomics Rare Diseases Bacterial Proteins Tandem Mass Spectrometry Insertional Metabolome medicine Molecular Biology Lung Chromatography Liquid Pyoverdine Agricultural and Veterinary Sciences Pseudomonas aeruginosa Articles Biological Sciences Complementation Mutagenesis Insertional Infectious Diseases Emerging Infectious Diseases Biochemistry chemistry Mutagenesis DNA Transposable Elements Phenazines Chromatography Liquid |
| Zdroj: | Journal of bacteriology, vol 196, iss 9 |
| Popis: | In microbiology, gene disruption and subsequent experiments often center on phenotypic changes caused by one class of specialized metabolites (quorum sensors, virulence factors, or natural products), disregarding global downstream metabolic effects. With the recent development of mass spectrometry-based methods and technologies for microbial metabolomics investigations, it is now possible to visualize global production of diverse classes of microbial specialized metabolites simultaneously. Using imaging mass spectrometry (IMS) applied to the analysis of microbiology experiments, we can observe the effects of mutations, knockouts, insertions, and complementation on the interactive metabolome. In this study, a combination of IMS and liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to visualize the impact on specialized metabolite production of a transposon insertion into a Pseudomonas aeruginosa phenazine biosynthetic gene, phzF2 . The disruption of phenazine biosynthesis led to broad changes in specialized metabolite production, including loss of pyoverdine production. This shift in specialized metabolite production significantly alters the metabolic outcome of an interaction with Aspergillus fumigatus by influencing triacetylfusarinine production. |
| Databáze: | OpenAIRE |
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