Identification of Thermosensory and Olfactory Neuron-Specific Genes via Expression Profiling of Single Neuron Types
| Autor: | Anne Lanjuin, Saikat Mukhopadhyay, Marc E. Colosimo, Aravinthan D. T. Samuel, Adam Brown, Christopher V. Gabel, Piali Sengupta |
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| Rok vydání: | 2004 |
| Předmět: |
Olfactory Nerve
Molecular Sequence Data Nerve Tissue Proteins Sensory system Biology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine medicine Animals Thermosensing Amino Acid Sequence Caenorhabditis elegans Caenorhabditis elegans Proteins Gene Cells Cultured Phylogeny Oligonucleotide Array Sequence Analysis 030304 developmental biology Neurons Genetics 0303 health sciences Agricultural and Biological Sciences(all) Reverse Transcriptase Polymerase Chain Reaction Biochemistry Genetics and Molecular Biology(all) Microarray analysis techniques Gene Expression Profiling Membrane Proteins Flow Cytometry Phenotype Sensory neuron Gene expression profiling medicine.anatomical_structure nervous system Homeobox General Agricultural and Biological Sciences Sequence Alignment Neuroscience 030217 neurology & neurosurgery Genetic screen |
| Zdroj: | Current Biology. 14:2245-2251 |
| ISSN: | 0960-9822 |
| DOI: | 10.1016/j.cub.2004.12.030 |
| Popis: | Most C. elegans sensory neuron types consist of a single bilateral pair of neurons, and respond to a unique set of sensory stimuli. Although genes required for the development and function of individual sensory neuron types have been identified in forward genetic screens, these approaches are unlikely to identify genes that when mutated result in subtle or pleiotropic phenotypes. Here, we describe a complementary approach to identify sensory neuron type-specific genes via microarray analysis using RNA from sorted AWB olfactory and AFD thermosensory neurons. The expression patterns of subsets of these genes were further verified in vivo. Genes identified by this analysis encode 7-transmembrane receptors, kinases, and nuclear factors including dac-1, which encodes a homolog of the highly conserved Dachshund protein [1]. dac-1 is expressed in a subset of sensory neurons including the AFD neurons and is regulated by the TTX-1 OTX homeodomain protein [2]. On thermal gradients, dac-1 mutants fail to suppress a cryophilic drive but continue to track isotherms at the cultivation temperature, representing the first genetic separation of these AFD-mediated behaviors. Expression profiling of single neuron types provides a rapid, powerful, and unbiased method for identifying neuron-specific genes whose functions can then be investigated in vivo. |
| Databáze: | OpenAIRE |
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