Construction of a shuttle lacmZα-based Escherichia coli-actinomycetes vector containing the phage JHJ-3 replicon

Autor:	L R, Gaudreau, C, Le Gouill, C V, Déry
Rok vydání:	1992
Předmět:	Genetic Vectors Chromosome Mapping Microbiology Streptomyces Lac Operon Genes Bacterial Actinomycetales Escherichia coli Genetics Bacteriophages Replicon Cloning Molecular Molecular Biology Plasmids
Zdroj:	FEMS Microbiology Letters. 99:257-260
ISSN:	0378-1097
DOI:	10.1016/0378-1097(92)90036-n
Popis:	Using the broad replicating range JHJ-3 phage replicon, a shuttle vector for Escherichia coli and actinomycetes has been constructed. The vector, pOJ31, bears the lacZ alpha fragment allowing a blue/white gene cloning system. pOJ31 also contains a polylinker of 15 unique cloning sites and the phage T7 promoter. The vector has been used to stably express the mel gene from plasmid pIJ702 in Streptomyces lividans.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::79224087c71608c776ea1ecaab63efb2 https://doi.org/10.1016/0378-1097(92)90036-n Zobrazit plný text záznamu Plný text