3D Cell Culture: Recent Development in Materials with Tunable Stiffness
| Autor: | Désirée Baruffaldi, Gianluca Palmara, Francesca Frascella, Candido Pirri |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Biomedical Engineering
Biocompatible Materials mechanical properties Biomaterials Extracellular matrix stiffness 3D cell culture Materials Testing medicine Humans Cell Culture Techniques Three Dimensional Particle Size tissue mimic hydrogels Chemistry Biochemistry (medical) Stiffness General Chemistry tunable materials Cell culture Self-healing hydrogels Biophysics Stress Mechanical medicine.symptom |
| Zdroj: | ACS Applied Bio Materials. 4:2233-2250 |
| ISSN: | 2576-6422 |
| DOI: | 10.1021/acsabm.0c01472 |
| Popis: | It is widely accepted that three-dimensional cell culture systems simulate physiological conditions better than traditional 2D systems. Although extracellular matrix components strongly modulate cell behavior, several studies underlined the importance of mechanosensing in the control of different cell functions such as growth, proliferation, differentiation, and migration. Human tissues are characterized by different degrees of stiffness, and various pathologies (e.g., tumor or fibrosis) cause changes in the mechanical properties through the alteration of the extracellular matrix structure. Additionally, these modifications have an impact on disease progression and on therapy response. Hence, the development of platforms whose stiffness could be modulated may improve our knowledge of cell behavior under different mechanical stress stimuli. In this review, we have analyzed the mechanical diversity of healthy and diseased tissues, and we have summarized recently developed materials with a wide range of stiffness. |
| Databáze: | OpenAIRE |
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