Modulation of the expression of alkaline phosphatase genes in a human malignant cell line passaged through nude mice
| Autor: | Denise Boccelli, Frances J. Benham, Gloria Balaban, Harry Harris |
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| Rok vydání: | 1981 |
| Předmět: |
musculoskeletal diseases
Cancer Research Immunodiffusion Hot Temperature Intestinal alkaline phosphatase Placenta Electrophoresis Starch Gel Mice Nude Isozyme HeLa Mice stomatognathic system Pregnancy Malignant cells Animals Humans Gene Fetus biology musculoskeletal neural and ocular physiology musculoskeletal system biology.organism_classification Alkaline Phosphatase Molecular biology Intestines Isoenzymes Oncology Biochemistry Gene Expression Regulation Alkaline phosphatase Female Neoplasm Transplantation HeLa Cells |
| Zdroj: | International journal of cancer. 28(3) |
| ISSN: | 0020-7136 |
| Popis: | Hep 2/5 cells, a clone of HeLa which expresses both placental-type alkaline phosphatase and fetal intestinal alkaline phosphatase (ALP), were grown as solid tumors in nude mice. Analysis of ALP isozymes present in tumor specimens excised at intervals over a 52-day period showed that levels of placental-type ALP had dropped markedly, while levels of the intestinal ALP remained fairly constant. Cells re-cultured from two two separate tumors regained most of the original placental ALP levels. However, levels of the fetal intenstinal ALP in cells returned to culture rapidly increased to more than six times the levels in the original cultured cells, and these increased levels persisted. Passage through nude mice thus induced at least semi-permanent changes in the level of expression of fetal intestinal ALP in Hep 2/5 cells, and the nature of these changes may relate to the role of the isozyme locus in malignant cells. |
| Databáze: | OpenAIRE |
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