Inhibition of trehalase affects the trehalose and chitin metabolism pathways in Diaphorina citri (Hemiptera: Psyllidae)
| Autor: | Huanan Su, Qin Zhang, Ci-Xiang Chen, Long Yi, Ba-Lian Zhong, Yu-Ling Huang, Yi-Ming Du, Hai-Zhong Yu, Zhan-Jun Lu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Diaphorina citri Chitin Genes Insect 01 natural sciences General Biochemistry Genetics and Molecular Biology Hemiptera 03 medical and health sciences chemistry.chemical_compound Animals Trehalase Gene Ecology Evolution Behavior and Systematics chemistry.chemical_classification biology Trehalose Validamycin biology.organism_classification 010602 entomology 030104 developmental biology Enzyme chemistry Biochemistry Gene Expression Regulation Insect Science Juvenile hormone RNA Interference Pest Control Agronomy and Crop Science Inositol |
| Zdroj: | Insect scienceReferences. 28(3) |
| ISSN: | 1744-7917 |
| Popis: | The Asian citrus psyllid, Diaphorina citri is the principal vector of huanglongbing, which transmits Candidatus Liberibacter asiaticus. Trehalase is a key enzyme involved in trehalose hydrolysis and plays an important role in insect growth and development. The specific functions of this enzyme in D. citri have not been determined. In this study, three trehalase genes (DcTre1-1, DcTre1-2, and DcTre2) were identified based on the D. citri genome database. Bioinformatic analysis showed that DcTre1-1 and DcTre1-2 are related to soluble trehalase, whereas DcTre2 is associated with membrane-bound trehalase. Spatiotemporal expression analysis indicated that DcTre1-1 and DcTre1-2 had the highest expression levels in the head and wing, respectively, and DcTre2 had high expression levels in the fat body. Furthermore, DcTre1-1 and DcTre1-2 expression levels were induced by 20-hydroxyecdysone and juvenile hormone Ⅲ, but DcTre2 was unaffected. The expression levels of DcTre1-1, DcTre1-2, and DcTre2 were significantly upregulated, which resulted in high mortality after treatment with validamycin. Trehalase activities and glucose contents were downregulated, but the trehalose content increased after treatment with validamycin. In addition, the expression levels of chitin metabolism-related genes significantly decreased at 24 and 48 h after treatment with validamycin. Furthermore, silencing of DcTre1-1, DcTre1-2, and DcTre2 reduced the expression levels of chitin metabolism-related genes and led to a malformed phenotype of D. citri. These results indicate that D. citri trehalase plays an essential role in regulating chitin metabolism and provides a new target for control of D. citri. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text