Simultaneous determination of the anticonvulsants, cinromide (3-bromo-N-ethylcinnamamide), 3-bromocinnamamide, and carbamazepine in plasma by high-performance liquid chromatography
| Autor: | L. J. Willmore, B. J. Wilder, Joseph Bruni, Robert J. Perchalski |
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| Rok vydání: | 1979 |
| Předmět: |
Reproducibility
Epilepsy Chromatography Chemistry medicine.medical_treatment Metabolite Extraction (chemistry) General Chemistry Carbamazepine Urine High-performance liquid chromatography chemistry.chemical_compound Anticonvulsant Cinnamates Reagent medicine Humans Anticonvulsants Female Chromatography High Pressure Liquid medicine.drug |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 163:187-193 |
| ISSN: | 0378-4347 |
| DOI: | 10.1016/s0378-4347(00)81462-0 |
| Popis: | A high-performance liquid chromatographic method is described for monitoring plasma concentrations of cinromide (3-bromo-N-ethylcinnamamide) and its de-ethylated metabolite. Carbamazepine levels can be easily measured by the same technique. The N-isopropyl analogue of cinromide is used as internal standard, and all compounds are easily separated on a reversed-phase column operated at 55 degrees with a small-diameter pre-column maintained at the same temperature. The extraction is rapid and generally applicable to plasma and urine samples that are to be analyzed by reversed-phase chromatography. Short- and long-term reproducibility studies show less than 4% relative standard deviation for replicate determinations for all drugs. Limits of quantitation are 10-20 ng/ml with an internal standard concentration of 3 micrograms/ml. Another metabolite of cinromide, 3-bromocinnamic acid, which may have some anticonvulsant effect, can be analyzed simultaneously by buffering the mobile phase and adding an ion-pairing reagent. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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