Toxoplasma gondii transmission by artificial insemination in sheep with experimentally contaminated frozen semen
| Autor: | Francisco Carlos Rodrigues de Oliveira, Andressa Ferreira da Silva, Juliana da Silva Leite, Angélica Consalter, Ana Maria Reis Ferreira, Edwards Frazão-Teixeira, Luis Fonseca Matos, Franciele Basso Fernandes Silva |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Sexual transmission medicine.medical_treatment Antibodies Protozoan Sheep Diseases Semen Biology Andrology Mice 03 medical and health sciences Food Animals Direct agglutination test parasitic diseases medicine Animals Small Animals Insemination Artificial Sheep Domestic Cryopreservation Sheep Equine Artificial insemination Toxoplasma gondii 030108 mycology & parasitology medicine.disease biology.organism_classification Toxoplasmosis Toxoplasmosis Animal Immunology biology.protein Female Animal Science and Zoology Histopathology Antibody Toxoplasma Semen Preservation |
| Zdroj: | Theriogenology. 90:169-174 |
| ISSN: | 0093-691X |
| Popis: | Toxoplasma gondii is a parasite considered one of the major causes of reproductive problems in sheep. Furthermore, the presence of the agent in ram semen urges the possibility of sexual transmission in this species. The aim of this study was to evaluate if ram's frozen semen spiked with T. gondii tachyzoites would be able to cause infection in sheep by laparoscopic artificial insemination (AI). Nine ewes tested seronegative to anti-T. gondii antibodies by the modified agglutination test (MAT) were superovulated and inseminated to collect embryos. Animals were divided into two groups: G1 (n = 5), ewes inseminated with semen containing 4 × 10(7) tachyzoites; and G2 (n = 4), ewes inseminated with tachyzoite-free semen (control group). To confirm infection, ewe's blood samples were collected on days -14, -7, 0, 7, 14, 21, 28, 35, 49 and 57 after AI for analysis by MAT and PCR. Tissue samples of these ewes were also collected for histopathology and immunohistochemistry (IHC). Seven days after AI, all ewes of group G1 had specific antibodies to T. gondii, while those of G2 were negative. Toxoplasma gondii DNA was detected in the blood of one ewe and parasites were observed in tissues of all five animals inseminated with contaminated semen, indicating that semen freezing protocol does not affect T. gondii transmission by artificial insemination in sheep. |
| Databáze: | OpenAIRE |
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