Corticotropin-releasing factor (CRF) can directly affect brain microvessel endothelial cells
| Autor: | Richard Letourneau, Pamela Esposito, Stanley Jacobson, Subimal Basu, Theoharis C. Theoharides |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
endocrine system
medicine.medical_specialty Time Factors Corticotropin-Releasing Hormone Blotting Western Central nervous system Stimulation Biology Western blot Pituitary Gland Anterior Internal medicine Cyclic AMP medicine Animals Tissue Distribution Antalarmin Receptor Molecular Biology Microvessel Cells Cultured Urocortins Ultrasonography Urocortin medicine.diagnostic_test General Neuroscience Brain Rats Endothelial stem cell Endocrinology medicine.anatomical_structure cardiovascular system Cattle Endothelium Vascular Neurology (clinical) hormones hormone substitutes and hormone antagonists Developmental Biology medicine.drug |
| Zdroj: | Brain Research. 968:192-198 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/s0006-8993(03)02237-6 |
| Popis: | Stress activates the hypothalamic–pituitary–adrenal (HPA) axis through release of corticotropin releasing factor (CRF), leading to production of glucocorticoids that down regulate immune responses. However, acute stress via CRF also has pro-inflammatory effects. We previously showed that acute stress increases rat blood–brain barrier (BBB) permeability, an effect involving brain mast cells and CRF, as it was absent in W/Wv mast cell-deficient mice and was blocked by the CRF-receptor antagonist, Antalarmin. We investigated if CRF could also have a direct action on brain microvessel endothelial cells (BMEC) isolated from rat and bovine brain. BMEC were cultured and identified by electron microscopy. Western blot analysis of cultured BMEC identified CRF receptor protein; stimulation with CRF, or it structural analogue urocortin (Ucn) showed that the receptor is functionally coupled to adenylate cyclase as it increased cyclic AMP (cAMP) levels by 2-fold. These findings suggest that CRF could affect BMEC structure or function, as reported for increased cAMP levels by other studies. It is, therefore, possible that CRF may directly regulate BBB permeability, in addition to any effect mediated via brain mast cells. |
| Databáze: | OpenAIRE |
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