Isolation and Characterization of Phosphate Solubilizing Microbes from Rock Phosphate Mines and their Potential Effect for Sustainable Agriculture
Autor: | Waqas Ahmed, Aftab Ahmad Sheikh, Sabir Hussain Shah, Asia Munir, Motsim Billah, Awais Qarni, Laila Jafri, Sadia Alam, Kouser Majeed Malik, Naeem Khan, Khadim Hussain |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
0106 biological sciences
Bacillus safensis compost Geography Planning and Development TJ807-830 Management Monitoring Policy and Law engineering.material TD194-195 maize 01 natural sciences Renewable energy sources 03 medical and health sciences chemistry.chemical_compound GE1-350 characterization Bacillus licheniformis 030304 developmental biology 0303 health sciences biology Environmental effects of industries and plants Renewable Energy Sustainability and the Environment Compost fungi food and beverages biology.organism_classification Phosphate DAP Environmental sciences Horticulture chemistry Phosphorite Diammonium phosphate Penicillium Bacillus firmus engineering rock phosphate isolation solubilization 010606 plant biology & botany |
Zdroj: | Sustainability Volume 13 Issue 4 Sustainability, Vol 13, Iss 2151, p 2151 (2021) |
ISSN: | 2071-1050 |
DOI: | 10.3390/su13042151 |
Popis: | Continuous application of phosphate (P) mineral to soil renders apatite addition during each crop growing season which is of great concern from a sustainable agriculture viewpoint. Use of efficient phosphate solubilizing microbes (PSB) is one of the most effective ways to solubilize this apatite mineral in the soil. The current study targeted hydroxyapatite mines to explore, isolate and characterize efficient P solubilizers to solubilize apatite in the soil. Efficiency of isolated microbes to solubilize rock phosphate (hydroxyapatite) and tri-calcium phosphate (TCP) as well as indole-3-acetic acid (IAA) and 1-aminocyclopropane-1-carboxylate deaminase (ACC) activity were tested. Identification and phylogenetic analysis of bacterial and fungal isolates were carried out by 16S rRNA and internal transcribed spacer (ITS) rDNA sequence analyses, respectively. The isolated bacterial strains were identified as Staphylococcus sp., Bacillus firmus, Bacillus safensis, and Bacillus licheniformis whereas fungal isolates were identified as Penicillium sp. and Penicillium oxalicum. Results showed that the impact of identified strains in combination with three phosphate fertilizers sources (compost, rock phosphate and diammonium phosphate (DAP)) was conspicuous on maize crop grown in pot. Both bacterial and fungal strains increased the P uptake by plants as well as recorded with higher available P in post-harvested soil. Penicillium sp. in combination with compost resulted in maximum P-uptake by plants and post-harvest soil P contents, compared to other combinations of P sources and bio-inoculants. Screening and application of efficient P solubilizers can be a better option to utilize the indigenous phosphate reserves of soil as well as organic amendments for sustainable agriculture. |
Databáze: | OpenAIRE |
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