Processing Bodies Oscillate in Neuro 2A Cells
| Autor: | Melisa Malcolm, Lucía Saad, Laura Gabriela Penazzi, Eduardo Garbarino-Pico |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cell type processing bodies GE1/Hedls Circadian clock RNA GRANULES MEMBRANELESS ORGANELLES lcsh:RC321-571 purl.org/becyt/ford/1 [https] 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine NEURO 2A membraneless organelles Organelle Circadian rhythm purl.org/becyt/ford/1.6 [https] lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry Neuro 2A Ribonucleoprotein Messenger RNA Chemistry RNA granules PROCESSING BODIES Brief Research Report DDX6/P54/RCK Cell biology CLOCK GE1/HEDLS 030104 developmental biology circadian rhythms Cytoplasm CIRCADIAN RHYTHMS 030217 neurology & neurosurgery Neuroscience |
| Zdroj: | Frontiers in Cellular Neuroscience CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET Frontiers in Cellular Neuroscience, Vol 13 (2019) |
| ISSN: | 1662-5102 |
| DOI: | 10.3389/fncel.2019.00487 |
| Popis: | Circadian rhythms are biological variables that oscillate with periods close to 24 h that are generated internally by biological clocks. Depending on the tissue/cell type, about 5–20% of genes are expressed rhythmically. Unexpectedly, the correlation between the oscillations of messengers and the proteins they encode is low. We hypothesize that these discrepancies could be because in certain phases of the circadian cycle some messengers could be translationally silenced and stored. Processing bodies (PBs) are membraneless organelles formed by ribonucleoprotein aggregates located in the cytoplasm. They contain silenced messengers and factors involved in mRNA processing. A previous work showed that the number of cells containing these mRNA granules varies when comparing two time-points in U2OS cell cultures and that these differences disappear when an essential clock gene is silenced. Here we evaluate whether PBs oscillate in Neuro2A cells. We analyzed in cell cultures synchronized with dexamethasone the variations in the number, the signal intensity of the markers used (GE-1/HEDLS and DDX6), and the area of PBs between 8 and 68 h post-synchronization. All three parameters oscillated with periods compatible with a circadian regulated process. The most robust rhythm was the number of PBs. These rhythms could be generated by oscillations in proteins that have been involved in the nucleation of these foci such as LSM1, TTP, and BRF1. The described phenomenon would allow to explain the differences observed in the temporal profiles of some messengers and their proteins and to understand how circadian clocks can control post-transcriptionally cellular functions. Fil: Malcolm, Melisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; Argentina Fil: Saad, Lucia Florencia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones y Transferencia de Villa María. Universidad Nacional de Villa María. Centro de Investigaciones y Transferencia de Villa María; Argentina Fil: Penazzi, Laura Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; Argentina Fil: Garbarino Pico, Eduardo. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina |
| Databáze: | OpenAIRE |
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