PTH [1-34]-induced alterations predispose the mandibular condylar cartilage to mineralization
| Autor: | Mara H O'Brien, Sumit Yadav, Alexandro Lima, T. Gutierrez, Ravindra Nanda, Eliane H Dutra |
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| Rok vydání: | 2017 |
| Předmět: |
Cartilage
Articular 0301 basic medicine medicine.medical_specialty X-ray microtomography Parathyroid hormone Orthodontics Osteoarthritis Bone remodeling Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine medicine Animals 030203 arthritis & rheumatology Chemistry Cartilage Mandibular Condyle Histology X-Ray Microtomography medicine.disease 030104 developmental biology medicine.anatomical_structure Endocrinology Otorhinolaryngology Parathyroid Hormone Sclerostin Female Surgery Bone Remodeling Oral Surgery Immunostaining |
| Zdroj: | Orthodontics & Craniofacial Research. 20:162-166 |
| ISSN: | 1601-6335 |
| DOI: | 10.1111/ocr.12157 |
| Popis: | Objective To study the effects of intermittent parathyroid hormone (PTH [1-34]) on the mandibular condylar cartilage (MCC) and subchondral bone in adult female mice. Materials and methods Twenty-two, 20-week-old female mice were used for in vivo experiments. The experimental mice (n=11) received daily intraperitoneal injections of PTH [1-34] for 3 weeks, while control mice (n=11) received intraperitoneal injections of 0.9% saline solution. Mice were euthanized and then micro-computed tomography (micro-CT); histology and immunostaining were carried out to assess the response. Results Intermittent PTH [1-34] led to early MCC breakdown and surface irregularities. Micro-CT analyses indicated that PTH [1-34] treatment led to increased bone volume fraction, tissue density and trabecular thickness, while decreasing the trabecular spacing. Histological analyses showed decreased proteoglycan secretion, increased bone turnover (TRAP staining) and increased mineralization. Furthermore, PTH [1-34] treatment showed increased apoptosis of the cells. Our immunohistochemistry showed increased expression of pSMAD158 in the MCC and subchondral bone with PTH administration, whereas sclerostin (SOST) expression was decreased. Conclusions Intermittent PTH [1-34] results in early mineralization of the MCC, which may result in cartilage degeneration. Our results identified a novel mechanism by which PTH [1-34] induces alteration in the microarchitecture of the MCC and the subchondral bone. |
| Databáze: | OpenAIRE |
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