Red‐ and Far‐Red‐Emitting Zinc Probes with Minimal Phototoxicity for Multiplexed Recording of Orchestrated Insulin Secretion
| Autor: | Junwei Zhang, Jingfu Sun, Chao Tang, Zhixing Chen, Yunxiang Wu, Huixia Ren, Yiwen Zhao, Liangyi Chen, Tianyan Liu, Xiaohong Peng, Shusen Wang, Shiyan Tong |
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| Rok vydání: | 2021 |
| Předmět: |
Morpholino
medicine.medical_treatment chemistry.chemical_element Zinc Catalysis Rhodamine chemistry.chemical_compound Insulin Secretion medicine Humans Fluorescent Dyes geography geography.geographical_feature_category Molecular Structure Rhodamines Insulin Far-red General Chemistry General Medicine Islet Fluorescence chemistry Biophysics Phototoxicity HeLa Cells |
| Zdroj: | Angewandte Chemie. 133:26050-26059 |
| ISSN: | 1521-3757 0044-8249 |
| DOI: | 10.1002/ange.202109510 |
| Popis: | Zinc biology, featuring intertwining signaling networks and of critical importance to human health, witnesses exciting opportunities in the big data era of physiology. Here, we report a class of red- and far-red-emitting Zn 2+ probes with K d values ranging from 190 nM to 74 μM, which are particularly suitable for real-time monitoring the high concentration of Zn 2+ /insulin co-released during vesicular secretory events. Compared to the prototypical rhodamine-based Zn 2+ probes, the new class exploits morpholino auxochromes which eliminates phototoxicity during long-term live recording from isolated islets. A Si-rhodamine-based Zn 2+ probe with high turn-on ratio (> 100), whose synthesis was enabled by a new route featuring late-stage N-alkylation, allowed simultaneous recording of Ca 2+ influx, mitochondrial signal, and insulin secretion in isolated mouse islets. The time-lapse multicolor fluorescence movies and their analysis, enabled by red-shifted Zn 2+ and other orthogonal physiological probes, highlight the potential impact of biocompatible fluorophores on the fields of islet endocrinology and system biology. |
| Databáze: | OpenAIRE |
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